Caffeine-induced expression of c-fos mRNA and NGFI-A mRNA in caudate putamen and in nucleus accumbens are differentially affected by the N-methyl-d-aspartate receptor antagonist MK-801 |
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| Affiliation: | 1. Department of Psychology, The Ohio State University, 1835 Neil Avenue, Columbus 43210, OH, USA;2. Department of Neuroscience, The Ohio State University, 1835 Neil Avenue, Columbus 43210, OH, USA;1. United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan;2. Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan;3. Department of Molecular Pathobiochemistry, Gifu University Graduate School of Medicine, Yanagido 1-1, Gifu 501-1193, Japan;4. Department of Medical Technology, School of Health Sciences, Gifu University of Medical Science, Nagamine Ichihiraga 795-1, Seki, Gifu 501-3892, Japan;5. Kazusa DNA Research Institute, Chiba 292-0818, Japan;1. Division of Biochemistry, Biophysics, and Structural Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA;2. Program in Microbial Pathogenesis and Host Defense, Department of Microbiology and Immunology, University of California School of Medicine, San Francisco, CA 94143, USA;3. Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;4. Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA |
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| Abstract: | Caffeine (100 mg/kg, i.p.) induces a rapid increase in the expression of mRNA for the immediate early genes (IEGs) c-fos and NGFI-A in rat striatum. We have examined how this response is affected by pretreatment with either the noncompetitive N-methyl-d-aspartate (NMDA) receptor antagonist MK-801 (1 and 3 mg/kg, i.p.), the competitive NMDA receptor antagonist D-CPP (6 mg/kg, i.p.), or the non-selective excitatory amino acid receptor antagonist kynurenic acid (300 mg/kg, i.p.). The two NMDA receptor antagonists significantly reduced the caffeine-induced expression of both c-fos mRNA and NGFI-A mRNA in the medial part of the caudate putamen. The effect was less pronounced in the lateral part of the caudate putamen. MK-801 caused an enancement of c-fos and NGFI-A mRNA expression in nucleus accumbens. Pretreatment with kynurenic acid caused no marked alterations in the caffeine-induced expression of c-fos mRNA and NGFI-A mRNA in any brain region. These findings suggest that glutamatergic transmission via NMDA receptors contributes to the induction of c-fos mRNA and NGFI-A mRNA by caffeine in striatum. In addition we show that MK-801 can either increase or decrease the caffeine effect of IEGs depending on the region studied. |
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