可乐定对小鼠体外淋巴细胞转化的影响

可乐定是肾上腺素能α_2受体激动剂,呈剂量依赖性地抑制小鼠体外淋巴细胞诱生抗体反应。本文研究了可乐定时ConA或PHA诱导小鼠淋巴细胞转化反应的影响。在培养开始或培养24小时后加入可乐定(10~(-8)～10~(-5)M),对ConA或PHA诱导的淋巴细胞转化均无明显影响。实验提示,在体外反应个T淋巴细胞和B淋巴细胞对可乐定的反应可能存在差别。

EFFECT OF GLONIDINE ON MURINE LYMPHOCYTE TRANS-FORMATION IN VITRO

A postulated suppression of T lymphocyte function by clonidine (an adrenogic receptor agonist) was explored by utilizing mitogen induced lymphocyte transformation of mouse spleen cells in vitro. Added at the onset of the culture or 24h later, clonidine (10-(?)-10-8 M ) did not suppress T lymphocyte transformation induced by ConA or PHA. It implies that there is a difference in the sensitivity for clonidine between T and B lymphocyte, as clonidine has been suggested to suppress the SRBC induced primary immune response of mouse spleen cells in a dose dependent manner.