Graves病患者外周血OX40和OX40L分子表达的研究

探讨OX40和OX40L分子在Graves病患者外周血T淋巴细胞和单核细胞上的表达及其在Graves病发病机制中的可能作用。采用流式细胞仪检测技术对50例初发Graves病患者和30例健康志愿者外周血T细胞上OX40和单核细胞上OX40L的表达水平进行分析,并对28例治疗前后病人淋巴细胞上OX40/OX40L的表达进行了比较。结果:与正常对照组比,初发Graves病患者外周血CD4+T细胞上OX40表达水平明显升高,而CD8+T细胞上OX40表达水平无显著变化;同时,还检测到单核细胞上OX40L表达水平也明显增加。治疗后,FT3(游离三碘甲腺原氨酸)值恢复至健康对照组表达范围的Graves病患者其CD4+T细胞上OX40和单核细胞上OX40L的表达均显著降低至正常水平;而FT3值仍偏高的患者单核细胞上OX40L的表达降低,但未降至健康对照组表达范围,CD4+T细胞上OX40的表达则无显著变化。Graves病患者外周血T细胞和单核细胞上分别存在OX40和OX40L的异常表达,且与治疗效果密切相关,提示OX40/OX40L信号在T细胞与单核细胞相互作用过程中可能有助于自身反应性T细胞的持续活化,从而参与Graves病的免疫发生发展。

The expression of OX40 and OX40L in the peripheral blood of Graves disease patients

To investigate the expression of OX40 and OX40L molecules in peripheral blood T lymphocytes and mononuclear cells in Graves’ disease and their possible roles in the pathogenesis.OX40 and OX40L expression on T cells and mononuclear cells were detected by flow cytometry in 50 cases of Graves’ disease patients and 30 healthy volunteers.Twenty eight samples from the patients were examined both before and after therapy to analyzed the changes of OX40/OX40L expression.Compare with the healthy control group,the expression of OX40 on CD4+ T cells and OX40L expression on monocytes in Graves’ disease patients was significantly upregulated,while OX40 on CD8+ T cells had no significant change in expression level.After therapy,the patients whose FT3 levels resumed normal,their OX40 expression on CD4+ T cell and OX40L expression on monocytes returned toward normal,those whose FT3 levels didn’t resume normal,OX40 and OX40L expression on CD4+ T cells and monocytes didn’t return toward normal.OX40 and OX40L were abnormally expressed on CD4+ T cells and mononuclear cells in the peripheral blood of Graves’ disease and their expressions were correlated with theraputic effects,suggesting that OX40/OX40L signal may contribute to the prolonged activation of T cells,which participate in the development of Graves’ disease.