DMD患者脐带血干细胞移植中的HLA配型

[目的]了解假肥大型肌营养不良症(Duchenne muscular dystrophy,DMD)患者接受脐血干细胞移植中HLA-A B DR不相合几率.[方法]用PCR反向序列特异性寡核苷酸(polymerase chain reaction-reverse sequence specific oligonucleotide,PCR-RSSO)杂交技术,在对30例DMD患者进行HLA-A B OR基因分开的基础上,将其与广东脐血库中的668份脐血干细胞HLA配型分析比较.[结果]13%(4/30)例患者找到HLA抗原全相合并满足移植细胞数要求的脐血干细胞;50%(15/30)例患者找到HLA抗原1个不相合并满足细胞数要求的剂血干细胞;20%(6/30)例患者找到HLA抗原0～1个不相合且细胞数达要求的2倍以上脐血干细胞.[结论]DMD患者寻找到合适的脐带血干细胞的几率较高,有利于对此病进行临床脐血干细胞移植.

HLA Matching on Cord Blood Stem Cell Transplantation of DMD Patient

To learn the mismatching rate of HLA-A B DR for DMD patients and pre-pare for the clinical transplantation of umbilical cord blood stem cells. 30 blood samples were genotyped with HLA-A B DR alleles by means of polymerase chain reaction-Reverse sequence specif-ic oligonucleotide, (PCR-RSSO). Then compare the HLA gene type of 30 DMD patients with those of 668 unrelated donors from Umbilical Cord Blood Center of Guangdong Province. There were 4 patients with zero mismatching(4/30,13%) , 15 patients 1 mismatching,(15/30,50%). Other 6 patients(6/30,20%) with 0 to 1 HLA antigen mismatching whose stem cells numbers are as 2 times as demanded, occupied 20% of the total. [Conclusion] The probability of getting proper stem cells of umbilical cord blood in this study is higher, which implies the good prospect of application of umbili-cal cord blood stem cells in the future clinical transplantation for this kind of patients.