过氧化亚硝基对不同肝细胞的损伤作用研究

目的　探讨过氧化亚硝基 (ONOO- )对人胚肝细胞株L 0 2细胞和肝母细胞瘤细胞株HepG2细胞的损伤差异。方法　运用四唑盐 (MTT)比色法、单细胞凝胶电泳和流式细胞术 3种方法检测过氧化亚硝基对两株肝细胞的细胞活性、DNA损伤、细胞凋亡的影响。结果　过氧化亚硝基对两株肝细胞均有细胞毒性 ,引起DNA损伤和细胞凋亡 ,损伤程度呈剂量依赖关系 (P <0 0 5 ) ,在各浓度组L 0 2细胞的损伤程度均略高于HepG2细胞 ,但两者统计学差异无显著性 (P >0 0 5 )。两株细胞周期变化趋势相一致。结论　过氧化亚硝基对正常和肝癌细胞均有DNA损伤、凋亡和影响细胞周期的作用 ,但两者差异无统计学意义。

Damage effects of peroxynitrite on different hepatocyte lines

Objective To investigate the damages of peroxynitrite (ONOO -) to L-02 and HepG2 cell lines.Methods Use MTT singe cell gel electrophoresis and flow cytometry was applied to detect the effects of peroxynitrite on cell viability, DNA impairment and cell apoptosis level in two cell lines. Results Peroxynitrite had cell toxicity on both cell lines. It caused DNA damages, cell apoptosis and cell cycle change. Furthermore, the damage level was proportional to the concentration of peroxynitrite. The damages were more serious in L-02 in every concentration level than that in HepG2, but no statistic difference between two cell lines. Conclusion Peroxynitrite could cause DNA damage, apoptosis and changed cell cycles in both normal and carcinoma cell lines through no statistical significant difference among them was found.