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| 肿瘤抑素诱导喉癌Hep-2细胞凋亡的实验研究 | |
| 引用本文： | 王超,姚鸿超,季文樾,金德均,刘兴汉.肿瘤抑素诱导喉癌Hep-2细胞凋亡的实验研究[J].中国现代医学杂志,2006,16(15):2280-2283. |
| 作者姓名： | 王超 姚鸿超 季文樾 金德均 刘兴汉 |
| 作者单位： | 1. 中国医科大学附属第二医院,耳鼻咽喉头颈外科,辽宁,沈阳,110001 2. 哈尔滨医科大学附属第二医院,耳鼻咽喉头颈外科,黑龙江,哈尔滨,150086 3. 哈尔滨医科大学生物化学与分子生物学教研室,黑龙江,哈尔滨,150086 |
| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
| 摘 要： | 目的探讨肿瘤抑素对体外培养的人喉癌Hep-2细胞系凋亡的影响。方法用MTT法和台盼蓝染色法观察肿瘤抑素对喉癌Hep-2细胞生长的抑制作用。实验分为对照组和治疗组．光镜、电镜观察细胞形态及超微结构变化，采用DNA琼脂糖凝胶电泳检测细胞凋亡的梯形条带，TUNEL标记法检测细胞凋亡情况。结果随着肿瘤抑素浓度的增加喉癌Hep-2细胞的存活率下降。在相同浓度下。随着时间延长存活细胞逐渐减少。电镜观察治疗组细胞出现明显凋亡，琼脂糖电泳结果可见治疗组产生了特征性的DNA梯形条带，TUNEL标记法也证实治疗组可见明显的棕黄色凋亡细胞。结论肿瘤抑素通过诱导凋亡对体外培养的人喉癌Hep-2细胞系产生杀伤作用。 |
| 关 键 词： | 肿瘤抑素 喉癌 细胞凋亡 |
| 文章编号： | 1005-8982（2006）15-2280-04 |
| 收稿时间： | 2005-09-27 |
| 修稿时间： | 2005-09-27 |
Tumstain-induced apoptosis in human laryngeal carcinoma Hep-2 cell |
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| WANG Chao,YAO Hong-chao,JI Wen-yue,JIN De-jun,LIU Xing-han.Tumstain-induced apoptosis in human laryngeal carcinoma Hep-2 cell[J].China Journal of Modern Medicine,2006,16(15):2280-2283. | |
| Authors: | WANG Chao YAO Hong-chao JI Wen-yue JIN De-jun LIU Xing-han |
| Institution: | 1.Department of Otorhinolaryngology Head and Neck Surgery, the Second Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R.Chino; 2.Department of Otorhinolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R.China; 3.Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China |
| Abstract: | Objective] To demonstrate the effect of tumstain on apoptosis of human laryngeal carcinoma Hep-2 cell. Methods] Hep-2 cell was used and the inhibiting effect of them were selected by MTT assay and Trypan blue stain. The subjects were divided into 2 groups: control group and treatment group. The histological and ultrastructural changes of cell were scored using light and electron microscopy. The DNA fragments and apoptosis index(AI) were observed using agarose gel electrophoresis and TUNEL. Results] Cell viabilities decreased with the increasing concentrations of tumstain. When the concentrations are the same, survival Hep-2 cell decreased with time.In treatment group, the obvious change of apoptosis, charactered DNA fragment and dyed brown cells could be seen by electron microscopy, agarose gel electrophoresis and TUNEL. Conclusion] Tumstain can kill the human laryngeal carcinoma Hep-2 cell in vitro. |
| Keywords: | tumstatin laryngeal carcinoma apoptosis |
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