家蝇对高效氯氰菊酯抗药性的选育研究

目的 通过室内选育,获得家蝇对高效氯氰菊酯抗性品系,探讨拟除虫菊酯对家蝇抗性形成机制、遗传规律.为延缓家蝇抗药性的产生,制定科学合理的抗性治理措施,更有效地防治家蝇提供科学依据.方法 抗性选育参照国标GB 13917.1～13917.8-92(农药登记卫生杀虫剂室内药效试验方法>以亚致死浓度高效氯氰菊酯对敏感品系家蝇逐代喷施.抗性测定用生物测定方法测定不同世代家蝇的LD50并计算抗性指数.结果 在高效氯氰菊酯的连续作用下,经过22代的选育,敏感品系家蝇在较短时间内对该种化学杀虫剂会产生较高的抗药性,形成抗性品系,抗性系数达到104.23倍.结论 家蝇抗性的发生、发展,实质上是杀虫剂对家蝇种群中具有抗性基因的个体做定向选择的结果,选择压力越大,抗性发展越快.施药方法对抗性的选育具有重要影响.

Study of the resistance of Musca domestica against beta-cypermethrin through selective breeding

Objective To determine the mechanisms of development and hereditary patterns of the resistance against pyrethroids in Musca domestica using beta-cypermethrin-resistant strain domestically produced by selective breeding,providing scientific basis for the establishment of practical management strategies to restrain the development of insecticide resistance for effective control of houseflies.Methods Beta-cypermethrin of median lethal concentration applied to each generation of susceptible M.domestica strains,...