| 两种pH值条件下CNBr活化Sepharose 4B偶联DSA方法的比较研究 |
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| 引用本文： | 王念跃,赵伟,李勇,黄宇峰,端木浩. 两种pH值条件下CNBr活化Sepharose 4B偶联DSA方法的比较研究[J]. 中国生化药物杂志, 2005, 26(6): 356-358 |
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| 作者姓名： | 王念跃 赵伟 李勇 黄宇峰 端木浩 |
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| 作者单位： | 1. 东南大学,医学院,附属南京第二医院,江苏,南京,210003
2. 南京军区南京总医院,江苏,南京,210002 |
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| 基金项目： | 南京市医学重点科技发展项目(No.ZKX0303) |
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| 摘 要： | 目的研究碱性、中性两种条件下CNBr活化Sepharose 4B偶联欧蔓陀罗凝集素(DSA)的效果。方法在两种偶联条件下,通过改变CNBr用量、活化时间以及温度等条件,观察DSA的偶联率指标。结果碱性条件下,活化15 min即使增加CNBr用量至300 mg/ml凝胶,固定化凝集素偶联率没有提高,DSA偶联率较低,在29.1%~50.9%之间;中性条件下,CNBr用量仅为碱性条件下的20%,活化温度控制在-10~-20℃可能获得较好的活化效果,DSA偶联率在78.8%~80.9%之间。结论中性条件下CNBr活化Sepharose 4B偶联DSA,实验易于控制,DSA偶联率比较稳定,实验重复性好于碱性条件下的结果,且可获较高偶联率的DSA-Sepharose 4B。
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| 关 键 词： | 亲和色谱 溴化氰 欧蔓陀罗凝集素 偶联 糖蛋白 |
| 文章编号： | 1005-1678(2005)06-0356-03 |
| 修稿时间： | 2005-05-23 |
The studied on coupling DSA with CNBr-activated Sepharose 4B under different pH condition |
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| WANG Nian-yue,ZHAO Wei,LI Yong,HUANG Yu-feng,DUANMU Hao. The studied on coupling DSA with CNBr-activated Sepharose 4B under different pH condition[J]. Chinese Journal of Biochemical Pharmaceutics, 2005, 26(6): 356-358 |
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| Authors: | WANG Nian-yue ZHAO Wei LI Yong HUANG Yu-feng DUANMU Hao |
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| Affiliation: | WANG Nian-yue~1,ZHAO Wei~1,LI Yong~2,HUANG Yu-feng~2,DUANMU Hao~1 |
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| Abstract: | PurposeTo study the effect of coupling DSA with CNBr-Activated Sepharose 4B under the alkaline and neutral condition.MethodsIt was observed the percentage of coupling DSA with CNBr-activated Sepharose 4B under variable conditions such as duration of CNBr activation,experiment temperature as well as dosages of CNBr udner different pH condition.ResultsUnder alkaline condition,the percentage of coupling DSA with CNBr-activated Sepharose 4B is between 27.0% and 50.9%, at the same time,under neutral condition,the percentage of coupling DSA with CNBr-activated Sepharose 4B is between 78.8% and 80.9%.ConclusionUnder neutral condition,the experiment is easy to be controlled,the percentage of coupling DSA with CNBr-activated Sepharose 4B is much higher than that under alkaline condition. |
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| Keywords: | affinity chromatography CNBr datura stramonium lectin coupling glycoprotein |
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