高表达的miR-301b和miR-769-5p对非小细胞肺癌A549细胞生长的影响

目的:探讨高表达的miR-301b和miR-769-5p对非小细胞肺癌A549细胞生长的影响。方法:采用实对荧光定量聚合酶链反应检测A549细胞中miR-301b和miR-769-5p的表达；使用 qRT-PCR检测增殖相关基因miRNA表达的变化，并将实验组miR-301b和miR-769-5p表达量上调(与阴性对照组比较)；采用MTT法检测并对比A549细胞增殖的改变；采用流式细胞仪检测并对比A549细胞周期以及凋亡的改变。结果:qRT-PCR结果表明，浸染了miR-301b和miR-769-5p过表达载体的A549细胞中miR-301b和miR-769-5p水平明显高于阴性对照组，差异有统计学意义（P＜0.01)。miR-301b过表达使G0/G1期的A549细胞增加，S和G2/M期的细胞减少；miR-769-5p过表达使G0/G1和G2/M期细胞增加，S期细胞减少。结论:miR-301b对靶基因FOXF2具有调控作用，miR-769-5p对靶基因ARID1A具有调控作用。miR-301b和miR-769-5p的过表达对肺癌A549细胞的细胞周期产生了明显影响，但没有影响细胞增殖和凋亡，miR-301b和miR-769-5p有可能成为肺癌分子靶向治疗调控效应靶点，可以进一步探索研究。

Effects of up-regulated miR-301b and miR-769-5p on cell growth in non-small cell lung cancer A549 cell line

Objective:To investigate the effects of miR-301b and miR-769-5p on cell growth in non-small cell lung cancer A549 cell line.Methods:A549 cells were transfected by lentiviral expression vector of miR-301b and miR-769-5p gene.Cell cycle and apoptosis were detected by flow cytometry and proliferation were detected by MTT assay after transfection.Results:The result of qRT-PCR showed that miR-301b and miR-769-5p expression in A549 cell infected by miR-301b and miR-769-5p were higher than others(P＜0.01).G0/G1 phase cycle was increased，S and G2/M phase cycle were arrested after up-regulation of miR-301b,S phase was arrested，G0/G1 and G2/M phase cycle were increased after up-regulation of miR-769-5p in A549 cell.Conclusion:miR-301b was validated to regulate FOXF2,and miR-769-5p modulated ARID1A.Additionally,overexpression of miR-301b and miR-769-5p affected cell cycle of A549 cells,but not cell proliferation and apoptosis.It may be helpful for further investigation of the regulation of the target effect for the molecular target therapy of lung cancer.