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Microbial Analysis in Primary and Persistent Endodontic Infections by Using Pyrosequencing |
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| Authors: | Bo-Young Hong Tae-Kwon Lee Sang-Min Lim Seok Woo Chang Joonhong Park Seung Hyun Han Qiang Zhu Kamran E. Safavi Ashraf F. Fouad Kee Yeon Kum |
| Affiliation: | ∗ Division of Periodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut;† School of Civil and Environmental Engineering, Yonsei University; WCU Center for Green Metagenomics, Yonsei University, Seoul, Republic of Korea;‡ Department of Conservative Dentistry, Jukjeon Dental Hospital, College of Dentistry, Dankook University, Jukjeon, Republic of Korea;§ Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea;‖ Department of Oral Microbiology and Immunology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Republic of Korea;¶ Division of Endodontology, Department of Oral Health and Diagnostic Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut;# Department of Endodontics, Prosthodontics and Operative Dentistry, Dental School, University of Maryland, Baltimore, Maryland;∗∗ Department of Conservative Dentistry, Dental Research Institute, Seoul National University Dental Hospital, Seoul National University School of Dentistry, Seoul, Republic of Korea |
| Abstract: | IntroductionThe aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections.MethodsPyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted.ResultsAnalysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P > .05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P > .05).ConclusionsThe present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections. |
| Keywords: | Diversity operational taxonomic unit persistent infection primary infection pyrosequencing richness |
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