薄层色谱-分光光度法测定黄芪粉针剂中黄芪甲苷的含量

目的：建立黄芪粉针剂的质量控制方法。方法：采用薄层色谱－分光光度法对黄芪粉针剂中的主要成分黄芪甲苷进行含量测定，展开剂为氯仿－甲醇－水（65：30：10），测定波长为418nm，结果：回归方程A＝1．538C＋0．189，r=0.9998，在80－240ug点样量范围内，吸光度与点样量呈良好的线性关系，平均回收率为98．68％，RSD＝2．52％（n=5)，结论：本方法使用简单仪器测定，结果可靠，专属性强，可作为黄芪粉针剂的质量控制标准。

Determination of content of astragaloside A in Astragalus membranaceus powder for injection by TLC-spectrophotometry

To develop a method for the quality control of Astrgalus membranaceus (Fisch.) Bge. powder for injection (AMPI). Methods　TLC-spectrophotometry was used to determine the content of astragaloside A in AMPI. The mobile phase was chloroform-methanol-water (65∶30∶10) and detected at the wave length of 418 nm. Results　The regression equation within the spotted range of 80～240 μg was A=1.538 C+ 0.189, with good linearity, r=0.999 8. The average recovery rate was 98.68% and RSD=2.52%(n=5). Conclusion　The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI.