Deep sequencing of Cotesia vestalis bracovirus reveals the complexity of a polydnavirus genome |
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| Authors: | Chen Ya-Feng Gao Fei Ye Xi-Qian Wei Shu-Jun Shi Min Zheng Hua-Jun Chen Xue-Xin |
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| Affiliation: | a Université de Lyon, Lyon, Franceb INRA, UMR754 Rétrovirus et Pathologie Comparée, UMS 3444/US8 Biosciences Gerland-Lyon Sud, Lyon, Francec Université Lyon 1, Lyon F-69007, Franced Ecole Nationale Vétérinaire de Lyon, Marcy L'étoile F-69000, Francee Ecole Pratique des Hautes Etudes, Lyon, Francef CNRS, Institut de Biologie et Chimie des Protéines, Lyon F-69007, France |
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| Abstract: | Integrase (IN) is the enzyme responsible for the integration of the retroviral genome into the host cell DNA. Herein, three mutants of conserved residues (V79, S85 and I146) of the central core domain (CCD) of an Avian Sarcoma/Leukemia Virus IN were analyzed in vitro. Our data revealed (i) the inability of S85T mutant to form dimers and tetramers in the absence of DNA and (ii) a slightly reduced ability of V79A IN in tetramers formation. Surprisingly, both mutants were still able to efficiently achieve concerted DNA integration. This could be explained by the ability of the two mutants to form complexes in the presence of DNA. These data suggest a strong structural role of the region encompassing V79 and S85 residues (β2/β3 turn-β3 strands) following binding to viral DNA and highlight the dynamic nature of IN. |
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| Keywords: | ASLV Retrovirus Integrase Concerted DNA integration Mutants |
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