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Chikungunya Virus Replication in Salivary Glands of the Mosquito Aedes albopictus |
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| Authors: | Anubis Vega-Rúa Christine Schmitt Isabelle Bonne Jacomine Krijnse Locker Anna-Bella Failloux |
| Affiliation: | 1.Arboviruses and Insect Vectors Unit, Department of Virology, Institute Pasteur, 25-28 rue du Docteur Roux, 75724 Paris cedex 15, France; (A.V.-R.);2.Ultrapole, Center for Innovation and Technology Research, Institute Pasteur, 25-28 rue du Docteur Roux, 75724 Paris cedex 15, France; (C.S.); (I.B.); (J.K.L.) |
| Abstract: | Chikungunya virus (CHIKV) is an emerging arbovirus transmitted to humans by mosquitoes such as Aedes albopictus. To be transmitted, CHIKV must replicate in the mosquito midgut, then disseminate in the hemocele and infect the salivary glands before being released in saliva. We have developed a standardized protocol to visualize viral particles in the mosquito salivary glands using transmission electron microscopy. Here we provide direct evidence for CHIKV replication and storage in Ae. albopictus salivary glands. |
| Keywords: | Chikungunya virus Aedes albopictus salivary glands budding replication transmission electron microscopy |