先天性肛门直肠畸形肛门内括约肌肌间神经节细胞及神经丛的研究

目的 通过对肛门直肠畸形动物模型肛门内括约肌肌间神经节细胞及神经丛数目的 观察,来研究其神经病理改变.方法 用乙烯硫脲诱导健康妊娠Wistar-Imammichi大白鼠产生67例有内括约肌的肛门直肠畸形动物模型,取这些胎仔,正中矢状面连续切片,经染色后观察直肠肛管发育情况及肛门内括约肌肌间神经节细胞和神经丛数的数目.结果 在实验组肛门直肠畸形鼠仔中每个视野的肛门内括约肌肌间神经节细胞数平均为1.206,每个视野的神经丛数平均仅为0.308个,而正常鼠仔平均值分别为3.710个和1.227个,经u检验(Z-test),两者比较差异均有统计学意义(P＜0.05、P＜0.01),而实验组正常鼠仔两项数值分别为3.012和1.187,与对照组相比差异无统计学意义.结论 肛门直肠畸形的病理改变涉及很多方面,包括肌肉、粘膜及神经系统.其中肛门内括约肌肌间神经节细胞数和神经丛数的减少是肛门直肠畸形的重要病理改变.

Study on the ganglion cell and the number of nerve plexus in the internal sphincter of the internal anus sphincter of rats with congenital anorectal malformations(ARM)

Objective To study the internal atlas sphincter of rats with congenital anorectal malformations(ARM)by observing the number of the ganglion cell and the number of nerve plexus in the internal sphincter.Methods Healthy pregnant Wistar- Imammichi rats are induced to ARM by ETU.67 rats with ARM were obtained.The pelvis was continuously cut from the exactly midsagittal plane, and the slices were stained with HE to observe the number of the ganglion cell and the number of nerve plexus in the internal sphincter under microscope.Result The average number of the ganglion cell in the rats with ARM was 1.206,and the number of the nerve pluxes is 0.310.The numbers in both two groups were extremely less than that in normal rats(P＜0.05).The rats without ARM which were also dealt with ETU had no distinctness compared with the normal rats.Conclusion The pathology changes of ARM includes many ways,for example,muscles,mucosa and the nerve system.The decrease of the ganglion cell and the nerve plexus in internal sphincter is an important aspect of ARM.