| Expression of Beclin1 in non small cell lung cancer and its clinical significance |
| |
| 引用本文： | Quan Liu,Jianjun Wang,Yongcheng Pan,Lifeng Meng,Xi Zhan. Expression of Beclin1 in non small cell lung cancer and its clinical significance[J]. 南京医科大学学报(自然科学版), 2008, 28(2): 121-124 |
| |
| 作者姓名： | Quan Liu Jianjun Wang Yongcheng Pan Lifeng Meng Xi Zhan |
| |
| 作者单位： | Quan Liu，Jianjun Wang，Yongcheng Pan，Lifeng Meng，Xi Zhan Department of Thoracic Surgery，Union Hospital，Huazhong University of Science and Technology，Wuhan 430022，China |
| |
| 摘 要： | Objective:To investigate the expression of autophagy related gene;Beclin1 in human non-small cell lung cancer(NSCLC)tissues.Methods:Protein expression of Beclin1 was determined by immunofluorescence staining and Western Blot,mRNA expression was analyzed by RT-PCR.Results:Immunofluorescence staining revealed that the level of Beclin1 expression in lung cancer was significantly lower than that in adjacent noncancerous tissues and normal tissues(expression rate 8.3%,P=0.000).The Beclin1 mRNA expression in lung cancer,adjacent noncancerous tissues and normal tissues was 1.372(±0.475)1.721(±0.521)and 1.553(±0.554)when F = 15.0,P < 0.01.Beclin1 protein expression in lung cancer,adjacent noncancerous tissues and normal tissues was 3.453(±0.852)5.423(±1.351)and 6.878(±0.997)F = 11.2,P < 0.01.Conclusion:The protein and mRNA expression of Beclin1 in lung cancer was much lower than those in and around cancer tissues and normal tissues,those differences having statistical significance.However in adjacent noncancerous tissues and normal tissues,the expression showed no difference.
|
| 关 键 词： | 自我吞噬 肺癌 细胞 基因 |
| 收稿时间： | 2007-10-15 |
Expression of Beclin1 in non small cell lung cancer and its clinical significance |
| |
| Quan Liu,Jianjun Wang,Yongcheng Pan,Lifeng Meng and Xi Zhan. Expression of Beclin1 in non small cell lung cancer and its clinical significance[J]. Acta Universitatis Medicinalis Nanjing, 2008, 28(2): 121-124 |
| |
| Authors: | Quan Liu Jianjun Wang Yongcheng Pan Lifeng Meng Xi Zhan |
| |
| Affiliation: | Department of Thoracic Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022,China |
| |
| Abstract: | Objective:To investigate the expression of autophagy related gene; Beclin1 in human non-small cell lung cancer(NSCLC) tissues. Methods:Protein expression of Beclinl was determined by immunofluorescence staining and Western Blot, mRNA expression was analyzed by RT-PCR. Results:Immunofluorescence staining revealed that the level of Beclinl expression in lung cancer was significantly lower than that in adjacent noncancerous tissues and normal tissues(expression rate 8.3%, P=0.000). The Bechnl mRNA expression in lung cancer, adjacent noncancerous tissues and normal tissues was 1.372 (± 0.475) 1.721 (± 0.521) and 1.553(± 0.554) when F=15.0, P ＜0.01. Beclinl protein expression in lung cancer, adjacent noncancerous tissues and normal tissues was 3.453(± 0.852) 5.423(±1.351) and 6.878(± 0.997) F=11.2, P ＜ 0.01. Conclusion:The protein and mRNA expression of Beclin 1 in lung cancer was much lower than those in and around cancer tissues and normal tissues, those differences having statistical significance. However in adjacent noncancerous tissues and normal tissues, the expression showed no difference. |
| |
| Keywords: | autophagy Beclin1 lung cancer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《南京医科大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《南京医科大学学报(自然科学版)》下载全文 |
