肾小球肾炎肾组织中HBV感染的标志

为了解肾组织中ＨＢＶＤＮＡ与ＨＢＶ抗原存在的关系，探讨肾组织中ＨＢＶ抗原的来源及其与病理变化的关系，我们检测２４６例肾炎肾组织中三种ＨＢＶ抗原。发现除肾小球沉积外，肾小管常有阳性表达。肾小管ＨＢｃＡｇ阳性率达２１．５４％，高于肾小球（１０．９８％）。有１８例肾组织经Ｓｏｕｔｈｅｒｎ印迹杂交检测ＨＢＶＤＮＡ，１５例阳性者中１４例肾组织ＨＢＶ抗原同时阳性，１２例血清感染标志亦阳性。结果提示某些肾炎可能与肾组织感染ＨＢＶ相关，肾组织中出现的ＨＢＶ抗原抗体免疫复合物除来自血循环外，有肾源性──原位形成的可能。

A study on the appearance of Hepatitis B virus markers in renal tissue of glomerulonephritis

BV antigens (HBCAg,HBsAg and HBeAg)in 246 cases of renal biopsy specimens with various types of glomerulonephritis were examined by immunohistochemical techniques. Southern blot hypridization was uxed to detect HBV DNA in 18 cases. The results showed that HBV antigens were frequently found in the tubular cells in addition to the appearance of HBV antigens on the glomeruli of certain glomerulonephritis. The positive rate of HBcAg in the tubular cells was 2l.54%,which is high-er than that of glomeruli(10. 98%).enal HBV DNA was positive in 15 cases and among them HBcAg expression in renal titqsue in 14 cases and HBV antigenemia in 12 cases. The results suggest that HBV may also ha implicated in the pathogenesis of certain cases of glomerulonephritis and the appearance of HBV antigen antibody immunocomplex in renal tissue, aside from originating in the circulation,there is the possibility of it originating from renal cells in situ.