氦氖激光对牙龈卟啉菌杀菌作用及形态影响的实验研究

目的：研究氦氖激光照射对牙龈卟啉菌杀菌及形态的影响，以期寻求治疗牙周炎的新手段。方法：用功率为6．3mW的氦氖激光照射牙龈卟啉菌，将细菌分为1个空白对照组和5个实验组。分别用不同照射时间（16、32、48、64、80min）的氦氖激光（波长632．8nm，功率6．3mW）进行照射，数活菌数和透射电镜下观察其形态学改变。结果：氦氖激光照射前后活细菌数无显著变化，但菌体的形态产生明显固缩。结论：单纯氦氖激光照射Pg，无明显的杀菌作用，但能使菌体形态发生变化。

Experimental Research on the Effect of He - Ne Laser on Porphyromonas Gingivalis

Objective:To find a new way for curing periodontitis through investigating the influence of He-Ne laser on porphyromonas gingivalis(Pg).Methods: Porphyromonas gingivalis(Pg) were irradiated by the He-Ne laser which had a power output of 6.3?mW.The bacterium of Pg were separated into 6 groups which including 5 experimental groups and 1 negative control group.The experimental groups were irradiated with He-Ne laser(wavelength: 632.8?nm,power of output: 6.3?mW) at different time(16?32?48?64?80?min).Living bacterium of Pg were counted after treated and the morphological changes of Pg were observed by transmission electron microscope.Result: The amount of living bacterium: there is no significant difference before and after irradiated by the He-Ne laser at different time.Transmission electron microscope: the longer time the bacterium were exposed under the He-Ne laser,the more significant shrinkage there was.Conclusion: There was no significant inhibition or killing effect of He-Ne laser on the bacterium of Pg.