| 急性分离大鼠心房肌细胞方法的改进及对钾、钠、钙电流的记录 |
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| 引用本文： | 龙毅,周亚南,殷跃辉.急性分离大鼠心房肌细胞方法的改进及对钾、钠、钙电流的记录[J].重庆医科大学学报,2011,36(8):910-912. |
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| 作者姓名： | 龙毅 周亚南 殷跃辉 |
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| 作者单位： | 重庆医科大学附属第二医院心血管内科,重庆,400010 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:介绍1种改进的成年SD大鼠心房肌细胞分离方法.方法:SD大鼠麻醉后,快速取出心脏,将主动脉结扎在Langendorff灌流装置上,无钙台式液灌流5 min后换用酶液灌流10～12 min,剪取心耳组织于KB液中剪碎并吹打,200目细胞筛网过滤,将细胞滤液500 r离心2 min,梯度复钙后将细胞悬液置于4℃冰箱中...
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| 关 键 词： | 膜片钳 心肌细胞 瞬时外向钾电流 钠电流 L型钙电流 细胞分离 |
A modified method for isolation of rat cardiomyocytes and recording of potassium,sodium and calium current |
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| LONG Yi,ZHOU Ya-nan,YIN Yue-hui.A modified method for isolation of rat cardiomyocytes and recording of potassium,sodium and calium current[J].Journal of Chongqing Medical University,2011,36(8):910-912. |
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| Authors: | LONG Yi ZHOU Ya-nan YIN Yue-hui |
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| Institution: | LONG Yi,ZHOU Ya-nan,YIN Yue-hui(Department of Cardiology,the Second Affiliated Hospital,Chongqing Medical University) |
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| Abstract: | Objective:To introduce a modified isolation method for acquiring rat atrial myocytes.Methods:The heart of adult sprague-dawley(SD) rat was rapidly excised after successful anesthesia and mounted on a Langendorff apparatus.It was perfused retrogradely via the aorta for five minutes with Ca2+-free Tyrode's solution and then perfused with collagenase dissolved in Tyrode's solution for ten to twelve minutes,and then the atrial appendage was sheared and stored in a kraftbruhe(KB) solution.Thereafter tissue was m... |
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| Keywords: | patch clamp atrial myocytes transient outward potassium current sodium current L-type calium current cell isolation |
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