| 结缔组织生长因子全长及其缺失突变体重组腺病毒的构建和鉴定 |
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| 引用本文： | 孙双双,陈红,唐敏,施琼,黎玉叶,王建伟,周兰,何通川,左国伟.结缔组织生长因子全长及其缺失突变体重组腺病毒的构建和鉴定[J].重庆医科大学学报,2011,36(8):949-953. |
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| 作者姓名： | 孙双双 陈红 唐敏 施琼 黎玉叶 王建伟 周兰 何通川 左国伟 |
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| 作者单位： | 1. 重庆医科大学临床检验诊断学教育部重点实验室
2. 重庆医科大学基础医学院组织学与胚胎学教研室,重庆,400016 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:结缔组织生长因子(Connective tissue growth factor,CTGF)含有4个不同功能结构域,构建表达带有3×Flag标签的全长基因及5个缺失突变体的重组腺病毒,以研究CTGF各结构域的功能和作用机制.方法:通过聚合酶链反应(Polymerasechain reaction,PCR)等克隆方...
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| 关 键 词： | 结缔组织生长因子 缺失突变体 重组腺病毒 |
Establishment of six adenoviruses that express the full length of connective tissue growth factor and its deletion mutants |
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| SUN Shuang-shuang,CHEN Hong,TANG Min,SHI Qiong,LI Yu-ye,WANG Jian-wei,ZHOU Lan,HE Tong-chuan,ZUO Guo-wei.Establishment of six adenoviruses that express the full length of connective tissue growth factor and its deletion mutants[J].Journal of Chongqing Medical University,2011,36(8):949-953. |
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| Authors: | SUN Shuang-shuang CHEN Hong TANG Min SHI Qiong LI Yu-ye WANG Jian-wei ZHOU Lan HE Tong-chuan ZUO Guo-wei |
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| Institution: | SUN Shuang-shuang1,CHEN Hong1,TANG Min1,SHI Qiong1,LI Yu-ye1,WANG Jian-wei2,ZHOU Lan1,HE Tong-chuan1,ZUO Guo-wei1(1.The Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,2.Department of Histology and Embryology,College of Basic Medicine,Chongqing Medical University) |
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| Abstract: | |
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| Keywords: | connective tissue growth factor deletion mutant recombinant adenovirus |
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