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| 体外纯化乳鼠许旺细胞的4种方法比较(英文) | |
| 引用本文： | 陈钢,杨彩虹,田林强,郭凤劲,陈安民,孙凯. 体外纯化乳鼠许旺细胞的4种方法比较(英文)[J]. 中国组织工程研究与临床康复, 2010, 14(10). DOI: 10.3969/j.issn.1673-8225.2010.10.039 |
| 作者姓名： | 陈钢 杨彩虹 田林强 郭凤劲 陈安民 孙凯 |
| 作者单位： | 1. 江西省人民医院骨一科,江西省南昌市,330006 2. 华中科技大学同济医学院附属同济医院骨科,湖北省武汉市,430030 |
| 基金项目： | 江西省自然科学基金资助项目 |
| 摘 要： | 背景:许旺细胞是外周神经系统中修复神经损伤及神经疾病的主要种子细胞之一.但是,许旺细胞的来源有限,且由于成纤维细胞的污染,使许旺细胞的纯度受到影响.有学者提出了其他的纯化方法,但每种方法都存在其不足之处而不能很好的满足临床应用的需要.目的:比较差速贴壁消化法、冷喷注法、磁珠分选法和G418筛选法4种体外纯化乳鼠许旺细胞方法的差异.方法:取SD大鼠坐骨神经得到所需神经组织.分别用差速贴壁消化法、冷喷注法、磁珠分选法和G418筛选法纯化许旺细胞.比较4种方法获得细胞的活力,免疫组织化学法鉴定许旺细胞并计算纯度.结果与结论:差速贴壁消化法所得到的细胞纯度稍低,活力尚可;冷喷注法获得的细胞纯度和活力均较高;磁珠分选法获得的细胞纯度和冷喷注法相当,但是活力稍差:G418筛选法活力差,纯度高. |
| 关 键 词： | 许旺细胞 差速贴壁消化 冷喷注 磁珠分选 G418筛选 |
Schwann cells purification by four different methods in vitro |
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| Chen Gang,Yang Cai-hong,Tian Lin-qiang,Guo Feng-jin,Chen An-min,Sun Kai. Schwann cells purification by four different methods in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2010, 14(10). DOI: 10.3969/j.issn.1673-8225.2010.10.039 | |
| Authors: | Chen Gang Yang Cai-hong Tian Lin-qiang Guo Feng-jin Chen An-min Sun Kai |
| Affiliation: | Chen Gang1,Yang Cai-hong2,Tian Lin-qiang2,Guo Feng-jin2,Chen An-min2,Sun Kai2 1First Department of Orthopedics,Jiangxi Provincial People's Hospital,Nanchang 330006,Jiangxi Province,China,2Department of Orthopedics,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,Hubei Province |
| Abstract: | BACKGROUND: Schwann cell is one of the major seed cells in peripheral nervous system and plays an important role in neural injury and neural disease. However, the source of Schwann cells is limited. And the purity of Schwann cells is affected due to the pollution of fibroblasts. Many purified methods have been proposed, but every one has its defect to satisfy the clinical demand. OBJECTIVE: To compare the differences among differential adhesion purified method, cold jet purified method, immunomagnetic beads... |
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