牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究

目的：研究牙周炎患者唾液一氧化氮（ＮＯ）的含量，及其与牙周病各临床指标的相关性。方法：选择成人牙周炎患者２３例为实验组，健康人２７例为对照组。记录牙周炎患者的牙龈出血指数（ＧＢＩ）、牙周袋深度（ＰＤ）和附着丧失量（ＡＬ）。ＮＯ的含量由检测唾液亚硝酸盐的含量来代表。测定实验组和对照组所有病例的唾液ＮＯ的含量。结果：对照组唾液ＮＯ的含量为２８．８０６±６．６０４μｍ／Ｌ，男女间无显著性差异。实验组唾液ＮＯ的含量为５５．３６１±１３．３１９μｍｏｌ／Ｌ，明显高于对照组。牙周炎患者唾液ＮＯ的含量与ＰＤ、ＡＬ间存在明显的正相关。结论：牙周炎患者唾液ＮＯ的含量显著高于健康人，并与牙周炎的严重程度有关。

The Investigation on Nitric Oxide Levels in Saliva and Their Relationship with the Severity of Periodontitis

OBJECTIVE: To study nitric oxide levels in saliva of patients with periodontitis and the relationship between nitric oxide levels and the severity of periodontitis. METHODS: Samples of saliva from 23 adult subjects with periodontitis and 27 healthy control subjects were collected at their first appointment. Data of gingival bleeding index (GBI), pocket depth (PD) and attachment level (AL) were recorded. Salivary nitrite was detected with Greiss method, which stood for the content of nitric oxide (NO). RESULTS: It was found that NO in saliva of patients with periodontitis were significantly higher than that in saliva of healthy individuals (P < 0.01). The levels of NO were 55.361 +/- 13.319 mumol/L and 28.806 +/- 6.604 microns/L in saliva with periodontitis and control respectively. Significant relationship was found between AL and salivary NO level (P < 0.02) and between PD and salivary NO level (P < 0.05). CONCLUSION: The change of salivary NO level is related with periodontitis.