柚皮素对ARPE-19和HUVEC细胞的抗氧化作用

目的：研究柚皮素对人视网膜色素上皮细胞（ARPE-19）和人脐静脉内皮细胞（HUVEC）的抗氧化作用。方法：采用MTT的方法检测ARPE-19和HUVEC细胞的生存率及增殖率。结果：3，10mg／L柚皮素能显著增加ARPE-19细胞的增殖率达10．8％和11．4％。10mg／L柚皮素能提高ARPE-19细胞在缺氧，0．3mmol／LNaN，及200μmol／L，H2O2条件下的生存率分别为55．2％，69．2％及50．3％。1mg／L柚皮素能提高ARPE-19细胞在50μmol／L t-BHP和30mg／LNaIO3条件下的生存率达20．2％和30．4％。30mg／L柚皮素能够促进ARPE-19细胞在50μmol／L t-BHP条件下的增殖率达32．2％，而1mg／L柚皮素可以提高30，100，300mg／LNaIO3处理的ARPE-19细胞的增殖率达30．3％，10．3％及18．5％。3，10及30mg／L柚皮素抑制HUVEC的增殖率分别为23．9％，70．4％及77．9％。1，3mg／L柚皮素能提高HUVEC细胞在缺氧条件下的生存率达10．7％和13．1％，以及提高在300mg／LNaIO3条件下的生存率达41．2％和37．7％。3mg／L柚皮素能提高HUVEC细胞在200，400μmol／L H2O2条件下的生存率达20．1％和21．5％．结论：柚皮素能够促进ARPE-19细胞的增殖率，抑制HU-VEC生长，同时对这两种细胞均有抗氧化作用。因此，柚臾素是治疗老年黄斑变性的很有前景的候选药物。

Antioxidant activity of naringenin on various oxidants induced damages in ARPE-19 cells and HUVEC

AIM: To evaluate the antioxidant activity of naringenin in human retinal pigment epithelium (ARPE-19) cells and human umbilical vein endothelial cells (HUVEC). ·METHODS: MTT assay was used to measure the viability and proliferation of ARPE-19 cells and HUVEC. ·RESULTS: Three and 10mg/L naringenin significantly increased the proliferation of ARPE-19 cells by 10.8% and 11.4%, respectively. Ten mg/L naringenin increased hypoxia-, 0.3mmol/L NAN3-, and 200μmol/L H2O2- induced damage of ARPE-19 cells by 55.2%, 69.2%, and 50.3%, respectively. One mg/L naringenin increased the viability of 50μmol/L t-BHP-, and 30mg/L NalO3-treated ARPE-19 cells by 20.2% and 30.4%, respectively. Thirty mg/L naringenin also increased the proliferation of 50μmol/L t-BHP-treated ARPE-19 cells by 32.2%, and 1mg/L naringenin increased the proliferation of 30, 100 and 300 mg/L NalO3-treated ARPE-19 cells by 30.3%, 10.3% and 18.5%, respectively. The reduction of HUVEC was 23.9%, 70.4% and 77.9% in the 3, 10 and 30mg/L naringenin-treated groups, respectively. Furthermore, 1 and 3mg/L naringenin increased hypoxia-induced damage in HUVEC by 10.7% and 13.1%, and 300mg/L NalO3- induced damage in HUVEC by 41.2% and 37.7%. Three mg/L naringenin increased 200 and 400μmol/L H2O2-in-jured HUVEC by 20.1% and 21.5%, respectively. ·CONCLUSION: Naringenin increases the proliferation of ARPE-19 cells and inhibites the growth of HUVEC, and has potent antioxidant activity in ARPE-19 cells and HUVEC.