莲须的遗传毒性研究

目的： 评价莲须的急性毒性及遗传毒性,为临床应用提供毒理学资料。 方法： 急性毒性：设21.50、10.00、4.64、2.15 g / kg 4剂量组灌胃。 Ames试验：设0.5、 5、 50、 500、 5 000 μg / 皿剂量,观察加或不加S 9混合物对TA 97、TA 98、TA 100、TA 102菌株的影响。小鼠骨髓嗜多染红细胞微核试验：设10.0、5.0、2.5 g / kg 3剂量组,2次灌胃后,6 h制片,镜检。小鼠精子畸形试验：设2.50、5.0、10.0 g / kg 3剂量,连续灌胃5 d,第35 d后制片、镜检。 结果： 急性毒性试验表明：莲须属于无毒级物质。Ames试验：为阴性结果,未发现对小鼠骨髓嗜多染红细胞有致突变作用和对小鼠精子有致突变作用。 结论： 莲须毒性较低,未发现有遗传毒性。

STUDY ON GENETIC TOXICITY OF STAMEN NELUMBINIS

Purpose: To provide toxicity data of Stamen Nelumbinis(SN) for clinic application. Methods:Acute toxicity: groups of mice for four doses(21.5, 10.0, 4.64, 2.15 g / kg) were administrated by gavage. Ames test: groups for five doses(0.5, 5, 50, 500, 5 000 μg / plate) were divided to observe the effects of SN on strains of TA 97, TA 98, TA 100, TA 102 with or without S 9 mixture added. Micronucleus test of bone marrow PCE cell in mice: Three groups for 2.5, 5.0, 10.0 g / kg were divided. After the animals received SN by gavage two times, they were killed. Then the slides were made and observed. Sperm shape abnormality test in mice: three groups for 0.5, 5.0, 10.0 g / kg were divided. The animals were killed on 35 d after they received SN by gavage for 5 d. The slides were made and observed. Results: SN was a substance with no toxicity according to the acute toxicity. Ames test: the result of SN was negative. SN had no mutagenesis on bone marrow PCE cells in mice, nor mutagenesis on sperm shape. Conclusion: The toxicity of SN was low and has not genetic toxicity.