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| 耐碳青霉烯类铜绿假单胞菌的产酶及联合药敏试验 | |
| 引用本文： | 叶明,杨喜民,王刚,雷光文. 耐碳青霉烯类铜绿假单胞菌的产酶及联合药敏试验[J]. 东南国防医药, 2011, 13(2): 135-137 |
| 作者姓名： | 叶明 杨喜民 王刚 雷光文 |
| 作者单位： | 解放军第三医院检验科,陕西宝鸡,721004 |
| 摘 要： | 目的研究耐碳青霉烯类铜绿假单胞菌(CR-PA)的产酶现状和抗菌药物的体外联合抗菌活性,为治疗CR-PA感染提供合理用药的实验依据。方法常规培养分离细菌,应用VITEK-Ⅱ全自动细菌分析仪鉴定细菌。常规药敏试验采用K-B纸片法,MIC测定采用琼脂平板倍比稀释法,按CLSI规定标准进行。结果从感染的标本中分离出190株铜绿假单胞菌,CR-PA占9.5%,均来自呼吸道标本。其中有10株同时产金属β-内酰胺酶(MBLs)、AmpC酶,4株仅产MBLs。头孢吡肟、头孢派酮/舒巴坦、亚胺培南与阿米卡星的协同率分别为33.3%、61.1%、27.8%。结论 CR-PA主要来源于呼吸道标本,其产酶率高,耐药性严重。建议临床对CR-PA引起的感染应使用含酶抑制剂复合药物(如哌拉西林/他唑巴坦、头孢派酮/舒巴坦)联合阿米卡星来治疗。 |
| 关 键 词： | 耐碳青霉烯类铜绿假单胞菌 抗生素 金属β-内酰胺酶 AmpC酶 最低抑菌浓度 联合用药 |
The analysis of zymogenic state against Carbapenems-resistant Pseudomonas aeruginosa using a susceptibility test |
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| YE Ming,YANG Xi-min,WANG Gang,LEI Guang-wen. The analysis of zymogenic state against Carbapenems-resistant Pseudomonas aeruginosa using a susceptibility test[J]. Journal of Southeast China National Defence Medical Science, 2011, 13(2): 135-137 | |
| Authors: | YE Ming YANG Xi-min WANG Gang LEI Guang-wen |
| Affiliation: | YE Ming,YANG Xi-min,WANG Gang,LEI Guang-wen.The Third Hospital of People's Liberation Army,Baoji,Shanxi 721004,China |
| Abstract: | Objective To analyze the zymogenic state and antimicrobial agent combination activity in vitro test of Carbapenems-resistant Pseudomonas aeruginosa(CR-PA),in order to provide laboratory data for clinical treatment.Methods All strains were isolated and identified by routine procedure and VITEK-Compact 2 automatic bacterial identification system.Routine antimicrobial susceptibility tests were performed with K-B method and MICs were detected with agar dilution method according to CLSI.Results There were 190 st... |
| Keywords: | CR-PA antimicrobial agent metallo-β-lactamases AmpC minimum inhibiting concentration（MIC） combining-using drug |
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