近红外光响应性复合纳米颗粒介导光热消融与自由基生成用于乳腺癌细胞的联合治疗

目的:制备新型聚多巴胺复合纳米颗粒,探究其对乳腺癌细胞的联合治疗.方法:以血清白蛋白,2,2-偶氮双[2-(2-咪唑啉-2-基)丙烷]二盐酸盐(AIPH),多巴胺为前驱物,通过一步氧化聚合法制备新型聚多巴胺纳米颗粒(AIPH@PB NPs);利用马尔文粒度分析仪、X射线光电子能谱和傅里叶红外光谱表征其理化学特性;利用紫...

Near-infrared light-responsive composite nanoparticles mediated photothermal ablation and free radical generation for combined inhibition of breast cancer cells

Objective: To develop novel polydopamine composite nanoparticles and explore its combined inhibition of breast cancer cells. Methods: Novel polydopamine nanoparticles -AIPH@PB NPs） were prepared by one-step oxidation polymerization using serum albumin（BSA）, 2,2-azobis［2 （2 imidazoline 2 yl）-propane- dihydrochloride （AIPH） and dopamine （DA） as precursors. The physical and chemical properties were characterized by Malvern particle size analyzer, X ray photoelectron spectroscopy（XPS） and Fourier infrared spectroscopy. Ultraviolet visible spectroscopy was used to characterize its ability to generate alkyl radicals. The photothermal conversion efficiency was characterized by near infrared thermal imager. The 4T1 breast cancer cells were used to verify the effect of tumor suppression in vitro. Results:  AIPH@PB NPs with good biocompatibility and a particle size of 165 nm were successfully prepared. Under the irradiation of 808 nm near infrared laser, AIPH@PB NPs could convert light energy into heat energy （photothermal conversion efficiency could be as high as 31%）. Meanwhile, local high temperature further induced the decomposition of AIPH to produce a large number of alkyl radicals. The cell experiment results showed that photothermal ablation and the generation of alkyl radicals mediated by AIPH@PB NPs significantly reduced the activity of 4T1 cells. Conclusion:  AIPH@PB NPs was successfully prepared and may， with strong photothermal conversion efficiency and high efficiency alkyl radical generation capacity, be used for combined inhibition of breast cancer cells.