Clara细胞分泌蛋白对创伤性休克肺损伤的保护作用

目的：探讨Clara细胞分泌蛋白（CC16）对创伤性休克大鼠模型继发性肺损伤的保护作用及其机制。方法：选择30只成年雄性SD大鼠,随机分为3组：假手术组、对照组、CC16处理组。采用创伤性休克动物模型,假手术组均完成所有手术操作,但不放血和复苏;对照组放血后在液体复苏前雾化吸入与CC16处理组等容量的生理盐水;CC16处理组在复苏前给予雾化吸入0.1μg/ml的重组人Clara细胞分泌蛋白（rh-CCSP）。实验结束后监测动脉血气分析及肺湿/干重比等指标变化,并测定肺组织中丙二醛（MDA）、髓过氧化物酶（MPO）及肺组织病理学的改变。结果：与对照组相比,CC16处理组的pH、PaO2值明显升高,BE值降低（P〈0.05）,肺湿/干重比减少（P〈0.05）;CC16处理组与假手术组间比较差异无统计学意义（P〉0.05）;与对照组比较CC16处理组的MDA、MP0含量明显降低,肺组织病理学形态也有明显改善。结论：雾化吸入rh-CCSP可减轻创伤性休克大鼠继发性肺损伤的炎症反应,对肺组织具有保护作用。

Protective effects of clara cell secretary protein on lung injury in traumatic shock

Objective：To investigate the effect and mechanisms of Clara cell secretary protein（CC16） on traumatic shock induced lung injury of rats.Method：Thirty SD male rats were randomly assigned to three groups： sham-surgery group,control group and CC16-treatment group.The animal model of traumatic shock was used in the research.Rats of sham-surgery group were operated,but not treated by depletion and recovery.Rats of control group inhaled after the physiological saline before fluid resuscitation.Rats of CC16-treatment group inhaled 0.1 μg/ml of recombinant human Clara cell secretary protein（rh-CCSP）before fluid resuscitation.Arterial blood gas analysis and wet/dry weight were detected.The lung tissue malondialdehyde（MDA）,myeloperoxidase（MPO）and lung tissue pathology change were observed.Result：Compared with control group,pH and PaO2 value in CC16-treatment group significantly increased.Meanwhile,BE value decreased and lung wet/dry weight ratio decreased（P0.05）.Compared with control group,contents of MDA and MPO in CC16-treatment group obviously decreased.Lung tissue pathology changed obviously.Conclusion：Inhalation of Rh-CCSP could have potential protective effect on lung tissues of rats with traumatic shock.