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| 重症急性胰腺炎合并细菌感染的快速诊断——附17例检测分析 | |
| 引用本文： | 邬林泉,熊志刚,吴柏华,邹书兵,邵江华. 重症急性胰腺炎合并细菌感染的快速诊断——附17例检测分析[J]. 新医学, 2008, 39(6) |
| 作者姓名： | 邬林泉 熊志刚 吴柏华 邹书兵 邵江华 |
| 作者单位： | 1. 江西南昌大学第二附属医院肝胆外科,330006 2. 江西丰城市人民医院外科,331100 |
| 摘 要： | 目的:探讨以16S rRNA基因为基础的细菌PCR法诊断重症急性胰腺炎早期合并细菌感染的价值.方法:17例疑有细菌感染的重症急性胰腺炎病人,术前B超引导下行胰周渗液细针穿刺检查,分别行以16S rRNA基因为基础的细菌PCR法检测和细菌培养.比较细菌PCR法检测结果和细菌培养结果.结果:17例中,PCR检测9例阳性,细菌培养10例阳性,该10例病人行手术治疗,术中所取胰周渗液行细菌培养证实为阳性.PCR检测重症急性胰腺炎合并细菌感染的敏感度为90%,特异度为100%.PCR法和细菌培养法分别需时5 h和3 d.结论:以16S rRNA基因为基础的细菌PCR法能快速、准确地诊断重症急性胰腺炎早期合并细菌感染,为手术治疗提供可靠依据. |
| 关 键 词： | 重症急性胰腺炎 细菌感染 聚合酶链反应 细针穿刺 诊断 |
Rapid diagnosis of severe acute pancreatitis complicated with bacterial infection |
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| Wu Linquan,Xiong Zhigang,Wu Bohua,et al.. Rapid diagnosis of severe acute pancreatitis complicated with bacterial infection[J]. New Chinese Medicine, 2008, 39(6) | |
| Authors: | Wu Linquan Xiong Zhigang Wu Bohua et al. |
| Affiliation: | Wu Linquan,Xiong Zhigang,Wu Bohua,et al.The Department of Hepatobiliary Surgery,The Second Affiliated Hospital of Nanchang University,Nanchang,330006,China |
| Abstract: | Objective:To explore the value of 16S rRNA gene based bacteria polymerase chain reaction(PCR) method in early diagnosis of severe acute pancreatitis with bacterial infection.Methods:The peripancreatic fluid of 17 patients with severe acute pancreatitis were obtained by ultrasound-guided percutaneous fine needle aspiration. The 16S rRNA gene based bacteria PCR assay and bacteria conventional culture were used to detect bacteria infection. The consequences of PCR assay and bacteria conventional culture were c... |
| Keywords: | Severe acute pancreatitis Bacterial infection Polymerase chain reaction Fine needle aspiration Diagnosis |
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