mB7－1基因转染小鼠黑色素瘤细胞诱导的抗瘤效应研究

目的：研究共刺激Ｂ７－１（ＣＤ６０）在诱导有效的抗肿瘤免疫反应反应中所起的作用。方法：在体外，三种肿瘤细胞与同上鼠脾淋巴细胞混合培养（ＭＬＴＣＳ）后，测定淋巴细胞增殖指数（ＭＴＴ法）和特异杀伤活性（ＬＤＨ释放改良法）；ＭＴＴＩ地测定肿瘤细胞体外增殖能力后，将Ｂ１６－ｎｅｏ和Ｂ１６－ｍＢ７－１妆种于Ｃ５７ＢＬ／６小鼠皮下后观察成瘤期、荷瘤小鼠存活以及肿瘤结节生长速度。结果：与野生型Ｂ１６细胞和模拟转

Antitumor Effect of Mouse B16 Melanoma Cells Transfected by mB7-1

Objective: To study the effect of B7-1 costimulatory molecule in inducing tumor immunity Methods: The lymphocytes were examined in vitro, for both proliferation indices (PI) and specific cytotoxic activity by MTT method and improved LDH-releasing method respectively, after 6 days of syngeneic mixed lymphocyte tumor cultures (MLTCs) After expansion, three B16 cell lines were tested by MTT method C57BL/6 mice were challenged subcutaneously either by the mock-transfected B16 cells (B16-neo) or mB7-1-transfected B16 cells (B16-mB7-1), following which the latency, survival time and tumor mass growth were noted Results: Compared with B16-wt and B16-neo cells, the B16-mB7-1 cells more effectively induced the proliferation of effector lymphocytes and the generation of specific lytic activity against B16-wt cells In vivo, the B16-mB7-1 cells demonstrated slower growth rate ( P < 0 05), longer latency and survival times ( P < 0 05) than B16-neo cells, although they showed similar proliferative activity in vitro Conclusion: The mB7-1 gene increased immunogenicity in poorly immunogenic tumor cells and at the same time decreased their tumorigenicity