转化生长因子β1基因-509位点多态性与重度慢性牙周炎易感性的关系

目的 探讨转化生长因子β1(transforming growth factor beta-1,TGF-β1)基因-509位点多态性与重度慢性牙周炎易感性的关系,以期从基因水平探讨牙周炎发病的遗传学机制.方法 用聚合酶链反应-限制性片段长度多态性方法检测102例重度慢性牙周炎患者(牙周炎组)和102名健康对照者(健康对照组)的TGF-β1基因-509位点,比较两组间此位点基因型分布和等位基因频率的差异.结果 TGF-β1基因-509位点CC、CT、TT基因型在牙周炎组和健康对照组的分布频率分别为44.1%(45/102)、47.1%(48/102)、8.8%(9/102)和29.4%(30/102)、51.0%(52/102)、19.6%(20/102),两组人群基因型分布频率差异有统计学意义(P＜0.05);等位基因C、T在牙周炎组和健康对照组分布频率分别为67.6%(138/204)、32.4%(66/204)和54.9%(112/204)、45.1%(92/204),两组人群的等位基因分布频率差异亦有统计学意义(P＜0.05),C等位基因携带者患重度慢性牙周炎的风险是T等位基因的1.718倍(OR=1.718,95%CI:1.148～2.569).结论 TGF-β1基因-509位点多态性与重度慢性牙周炎的发病具有相关性,C等位基因可能是重度慢性牙周炎的遗传易感基因.

Relationship between transforming growth factor beta-1 gene-509C/T polymorphism and severe chronic periodontitis

Objective To investigate the relationship between transforming growth factor beta-1 (TGF-β1 )gene-509C/T polymorphism and severe chronic periodontitis in Chinese Hans population.Methods TGF-β1-509C/T genotype polymorphism was analyzed in 102 patients with severe chronic periodontitis(periodontitis group) and 102 healthy controls( control group) by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method.Results The distributions of TGF-β1-509C/T genotype and allele were significantly different between severe chronic periodontitis group and control group ( P ＜ 0. 05 ). TGF-β1-509CC, CT and TT genotype frequency were 44. 1% ( 45/102 ), 47. 1%(48/102) ,8. 8% ( 9/102 ) in periodontitis group and 29. 4% ( 30/102 ), 51.0% ( 52/102 ), 19. 6%(20/102) in control group, respectively. The relative risk analysis found that C allele carriers had higher risk of suffering from severe chronic periodontitis compared with T allele carriers( OR = 1. 718,95% CI:1. 148 ～ 2. 569 ).Conclusions TGF-β1-509C/T polymorphism is associated with severe chronic periodontitis in Chinese Hans population, and C allele may be an important genetic susceptibility gene for severe chronic periodontitis.