穴位注射“冰片液”对血脑屏障通透性的影响

目的 :探讨“冰片液”穴位注射对SD大鼠血脑屏障通透性的影响 ,为临床提高某些中枢神经治疗药疗效提供实验依据。方法 :采用“哑门”穴位注射自制“冰片液” ,观察不同浓度“冰片液”穴位注射对伊文斯蓝 (Even’sBlue,EB)透过血脑屏障的作用 ,并与电针刺激组比较。结果 :“冰片液”穴位注射和电针刺激均可促进EB透过血脑屏障 (P <0 0 0 1 ,P <0 0 5) ,穴位注射“冰片液”高、低剂量组均优于电针组 (P <0 0 5) ,但“冰片液”高、低剂量组间无明显差异 (P >0 0 5)。结论 :“冰片液”穴位注射是促血脑屏障开放的一种有效方法 ,其作用优于电针刺激。

Effect of Acupoint Injection of Camphol-fluid on the Permeability of Blood-brain Barrier in Rats

Objective:To explore actions of acupoint injection of Camphol on the permeability of blood-brain barrier and the relevant mechanisms.Methods:30 SD rats were randomly divided into acupoint injection group 1(low dosage, AI-1 group), acupoint injection group 2(high dosage, AI-2 group), electroacupuncture (EA) group."Yamen"(GV 15) was used for injection of Camphol(0.4 g/kg and 0.8 g/kg), and "Baihui" (GV 20) was used for EA stimulation (50 Hz, 5 mA, for 15 min, once again 5 hr later). After treatment, the rats were killed for taking brain tissues that were then processed into supernate for detecting the permeability of blood-brain barrier of rats with spectrophotometric analysis. Results:After treatment, groups can promote the permeability of blood-brain barrier in rats, but the contents of Even's Blue in the brain tissues in the 3 treatment groups all significantly higher than that of control group (P<0.05～0.001), suggesting that acupoint injection and EA can strengthen the permeability of blood-brain barrier to Even's Blue. Comparison among the 3 treatment groups showed that the contents of Even's Blue of AI-1 and AI-2 groups were markedly higher than that of control group (P<0.05). Conclusion:Acupoint injection of Camphol can effectively improve the permeability of blood-brain barrier, which is better than that of electroacupuncture.