|
|||||
|
|
| 间充质干细胞移植对大鼠脑缺血区GDNF表达的影响 | |
| 引用本文： | 钱佳利,徐忠信,常颖,莽靖,王晓明,李昕华,郭丽. 间充质干细胞移植对大鼠脑缺血区GDNF表达的影响[J]. 中国实验诊断学, 2007, 11(9): 1140-1142 |
| 作者姓名： | 钱佳利 徐忠信 常颖 莽靖 王晓明 李昕华 郭丽 |
| 作者单位： | 1. 长春中医药大学,生理教研室,吉林,长春,130031 2. 吉林大学中日联谊医院,神经内科 3. 吉林大学公共卫生学院,毒理学教研室 |
| 基金项目： | 吉林省科技厅自然科学基金 |
| 摘 要： | 目的观察骨髓间充质干细胞移植对大鼠大脑中动脉栓塞后神经功能的影响及GDNF的表达。方法采用线栓法大鼠大脑中动脉栓塞(MCAO)模型,颈内动脉移植MSCs,Bederson神经功能评分观察大鼠神经功能,免疫组织化学和原位杂交技术检测GDNF表达。结果MSCs移植可显著提高大鼠局灶性脑缺血后神经功能,缺血周边区GDNF蛋白及mRNA表达水平均高于对照组。结论MSCs移植可促进神经功能恢复,可能通过增加GDNF的表达起到脑保护作用。 |
| 关 键 词： | 间充质干细胞 脑缺血 胶质细胞源性神经营养因子 |
| 文章编号： | 1007-4287（2007）09-1140-03 |
| 修稿时间： | 2007-02-12 |
Effect of transplantation of mesenchymal stem cells to GDNF after cerebral ischemia in rats |
|
| QIAN Jia-li, XU Zhong-xin CHANG Ying,et al.. Effect of transplantation of mesenchymal stem cells to GDNF after cerebral ischemia in rats[J]. Chinese Journal of Laboratory Diagnosis, 2007, 11(9): 1140-1142 | |
| Authors: | QIAN Jia-li XU Zhong-xin CHANG Ying et al. |
| Affiliation: | 1. Changchun University of Tradtiolwl Chimse Medicine, Changchzm 130031, China ; 2. Department of Neurol- ogy China-Japan Union Hospital, Jilin University; 3. Deportment of Toxicology, School of Public Health, Jilin University |
| Abstract: | Objective To observe the neurological functional recovery after transplanting mesenchymal stem cells(MSCs) to rats with middle cerebral artery occlusion(MCAO),and detect the expression of GDNF.Methods MCAO model was established with suture emboli method,measure functional outcome with Bederson neurological severity score.MSCs were transfered into rats through arteria carotis internal 24 hours after MCAO,detect expression of GDNF protein and mRNA by immunohistochemical and in situ hybridization method.Results MSCs transplantation could significantly improve the rats neurological functional recovery after MCAO.The expression of GDNFmRNA and protein is higher than the control group.Conclusion MSCs can significantly improve neurological recovery after MCAO in rats and protect the brain by increased GDNF expression. |
| Keywords: | Mesenchymal stem cells Cerebral ischemia Glial cell line derived neurotrophic factor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |