大肠癌中p53和PCNA表达水平的研究

目的 :研究大肠癌p53蛋白和增殖细胞核抗原 (PCNA)的表达及与肿瘤的浸润、转移的关系。方法 :应用免疫组化SP法 ,检测并观察 6 4例大肠癌p53和PCNA的表达水平。结果 :大肠癌中 p53阳性率为 57.8% ;p53表达水平与性别、年龄及肿瘤部位、组织学类型和肿瘤分化程度无关 (P >0 .0 5) ;与浸润深度和淋巴结转移有关 (P <0 .0 5)。大肠癌中PCNA阳性率为 85.9% ;PCNA表达水平与肿瘤组织学类型、分化程度、浸润深度和淋巴结转移有关 (P <0 .0 5)。结论 :p53和PCNA过表达在大肠癌浸润转移和细胞增殖过程中起重要作用 ,检测 p53和PCNA对于判断大肠癌的恶性程度、预测其淋巴结转移趋势有重要价值。

Expression of p53 and PCNA in Colorectal Carcinomas

Objective:To explore expression levels of p53 and PCNA in colorectal carcinomas and associated with carcinoma infiltration and metastasis.Methods:We detected expression of p53 and PCNA from 64 colorectal carcinomas specimens by immunohistochemistry technique.Results:The positive rate of p53 protein is 57.8%in colorectal carcinomas.The positive rate of p53 protein is related to carcinoma infiltration,lymph node metastasis(P<0.05),but not associated with sex,age,tumor site,histologic type,tumor differentiation(P>0.05).The positive rate of PCNA is 85.9%in colorectal carcinomas.The positive rate of PCNA is related to tumor histologic type,differentiation,infiltration and lymph node metastasis(P<0.05).Conclusions:It is important for infiltration,metastasis and cell proliferation that p53 and PCNA express in colorectal carcinomas.Detection of p53 and PCNA is valuable for judging malignant grade and predictiong lymph node metastasis.