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| HPLC法测定桔贝合剂中黄芩苷的含量 | |
| 引用本文： | 王海宁,杨翰. HPLC法测定桔贝合剂中黄芩苷的含量[J]. 中国药房, 2007, 18(27): 2132-2133 |
| 作者姓名： | 王海宁 杨翰 |
| 作者单位： | 1. 贵州省药品检验所,贵阳市,550004 2. 贵阳市第二人民医院,贵阳市,550005 |
| 摘 要： | 目的:建立以高效液相色谱法测定桔贝合剂中黄芩苷含量的方法。方法:色谱柱为Spherisorb C18(200mm×4.6mm,5μm),流动相为甲醇-水-磷酸(47:53:0.2),检测波长为280nm,流速为1mL.min-1,柱温为室温。结果:黄芩苷进样量在0.1024～0.9216μg范围内与峰面积积分值呈良好线性关系;平均加样回收率为99.98%,RSD=0.95%(n=6)。结论:本方法精密度高、分离度好,可用于桔贝合剂的质量控制。 |
| 关 键 词： | 黄芩苷 高效液相色谱法 桔贝合剂 含量测定 |
| 文章编号： | 1001-0408（2007）27-2132-02 |
| 收稿时间： | 2007-01-07 |
| 修稿时间： | 2007-05-27 |
Determination of Baicalin in Jubei Oral Liquid by HPLC |
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| WANG Haining,YANG Han. Determination of Baicalin in Jubei Oral Liquid by HPLC[J]. China Pharmacy, 2007, 18(27): 2132-2133 | |
| Authors: | WANG Haining YANG Han |
| Abstract: | OBJECTIVE:To determine the content of Baicalin in Jubei oral liquid by HPLC.METHODS:HPLC was adopted with Spherisorb C18(200mm× 4.6mm,5μ m) with column at room temperature.The mobile phase consisted of methanol-water-phosphoric acid(47:53:0.2) with a flow rate of 1mL· min-1.AUV-detector at 280nm was used.RESULTS:Baicalin showed good linear relationship in the range of 0.102 4～ 0.921 6μg.The average recovery was 99.98%,RSD=0.95%(n=6).CONCLUSION:The method is of high precision and good separation,it can be used for the quality control of Jubei oral liquid. |
| Keywords: | Baicalin HPLC Jubei oral liquid Content determination |
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