microRNA-137基因多态性与缺血性卒中后抑郁的相关性

目的分析microRNA-137基因多态性与缺血性卒中后抑郁的相关性。方法2017年1月至2019年1月,SNaPshot检测缺血性卒中后抑郁患者250例(患者组)和健康体检者250例(对照组)microRNA-137基因rs1625579位点的基因型,逆转录荧光定量聚合酶链反应检测外周血单个核细胞microRNA-137相对表达量,并分析其与rs1625579位点不同基因型的关系。结果患者组rs1625579位点等位基因T高于对照组(OR=2.033,95%CI 1.255~3.294,P=0.003),基因型TT高于对照组(OR=2.139,95%CI 1.272~3.595,P=0.004);患者组microRNA-137相对表达量显著低于对照组(t=28.230,P<0.001);对照组TT基因型的microRNA-137相对表达量显著低于GT+GG基因型(t=3.764,P<0.001)。结论microRNA-137基因rs1625579位点TT基因型可能通过下调microRNA-137的表达,参与缺血性卒中后抑郁的发病。

Association between microRNA-137 Gene Polymorphisms and Ischemic Post-stroke Depression

Objective To analyze the relationship between microRNA-137 gene polymorphisms and ischemic post-stroke depression (PSD).Methods January, 2017 to January, 2019, the single nucleotide polymorphism (SNP) of rs1625579 in microRNA-137 was genotyped in 250 ischemic PSD patients and 250 healthy controls with SNaPshot. The expression of microRNA-137 was measured with quantitative real-time polymerase chain reaction in the mononuclear cells, and the association of the SNP rs1625579 with microRNA-137 expression was analyzed.Results The allele T was more in the patients than in the controls (OR = 2.033, 95%CI 1.255 to 3.294, P= 0.003), as well as the genotype TT (OR = 2.139, 95%CI 1.272~3.595, P= 0.004). The microRNA-137 expression was less in the patients than in the controls (t = 28.23, P< 0.001). For the controls, the microRNA-137 expression was also less in those with genotype of TT than with GT and GG (t = 3.764, P< 0.001).Conclusion The genotype TT of SNP rs1625579 in microRNA-137 is a risk factor of susceptibility to ischemic PSD, which may associate with the decrease of expression of microRNA-137.