| Beclin 1在乳腺浸润性导管癌组织中的表达 |
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| 引用本文： | 姚维深,张旭,刘艳,周国华. Beclin 1在乳腺浸润性导管癌组织中的表达[J]. 河北医学, 2014, 0(7): 1057-1061 |
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| 作者姓名： | 姚维深 张旭 刘艳 周国华 |
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| 作者单位： | 姚维深 (南方医科大学附属南海医院胸外科,广东 佛山,528200)； 张旭 (中山大学肿瘤防治中心胸外科,广东 广州,510060)； 刘艳 (南方医科大学附属南海医院胸外科,广东 佛山,528200)； 周国华 (南方医科大学附属南海医院胸外科,广东 佛山,528200)； |
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| 基金项目： | 广东省佛山市医学类科技攻关项目，(项目编号：编号201208135) |
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| 摘 要： | 目的：探讨Beclin 1在乳腺浸润性导管癌中的表达及临床意义。方法：免疫组织化学EnVision 法、RT-PCR法和Western blot 法检测50例乳腺浸润性导管癌及相应癌旁组织Beclin 1表达，分析其临床意义。结果：三种方法联合检测发现癌组织中Beclin 1阳性率低于癌旁组织，癌组织中Beclin 1相对表达量也低于癌旁组织；癌组织中Beclin 1表达与患者病理分级、临床分期和淋巴结转移情况有关（P＜0．05），与患者年龄、绝经状况及乳头侵犯状况无关（P＞0．05）。结论：Beclin 1在乳腺浸润性导管癌中存在缺失表达或低表达，并与乳腺癌的发生、发展相关。
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| 关 键 词： | 乳腺浸润性导管癌 Beclin 1 免疫组织化学 RT-PCR Western blot |
The Expression of Beclin 1 in Invasive Ductal Carcinoma of the Breast |
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| Affiliation: | YAO Weishen, et al ( Nanhai Hospital Affiliated to Nanfang Medical University, Guangdong Foshan 528200, China} |
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| Abstract: | Objective：To investigate the expression of Beclin 1 in invasive ductal carcinoma （ IDC ） of the breast, and to evaluate the clinical implications.Method： Immunohistochemical, RT-PCR and Western blot methods were used to determine the expression of Beclin 1 in 50 cases of IDC and nearby mucosa of the carcinoma .The relationship between the gene expression and clinicopathological features were analyzed .Result：The lost and negative expression rates of Beclin 1 in IDC was higher than nearby mucosa of the carcinoma.The expression of Beclin 1 were negatively correlated with the clinicopathological features , tissue types and neck lymph node metastasis in IDC （P〈0.05）, and no correlated with ages, menopausal status and nipple infringement status （ P〉0.05）.Conclusion： There was a common lost and negative expression rates of Beclin 1 in IDC.The expression of Beclin 1 may relate to the tumorigenesis and development of the invasive ductal breast cancer . |
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| Keywords: | Invasive ductal carcinoma Beclin 1 Immunohistochemical RT-PCR Western blot |
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