羊栖菜多糖对S180荷瘤小鼠红细胞相关生化功能影响的研究

 目的分析羊栖菜多糖对S₁₈₀荷瘤小鼠红细胞相关生化功能影响的研究。方法建立肿瘤动物模型,分高、中、低剂量腹腔给予羊栖菜多糖7 d,采集红细胞,制备红细胞悬液,运用激光共聚焦扫描技术测定荷瘤小鼠红细胞内Ca²⁺]的变化;运用相关试剂盒测定红细胞膜表面唾液酸含量和Na⁺,K⁺-ATPase及Ca²⁺,Mg²⁺-ATPase活性的变化;用流式细胞仪分析红细胞膜电位水平的变化;采用高效毛细管电泳法测定红细胞电泳合淌度的变化。结果羊栖菜多糖能降低荷瘤小鼠红细胞内Ca²⁺],升高膜表面唾液酸的含量,增强膜表面Na⁺,K⁺-ATPase及Ca²⁺,Mg²⁺-ATPase的活性,提高红细胞膜电位水平,提高红细胞的电泳合淌度。结论羊栖菜多糖可调节或恢复S₁₈₀荷瘤小鼠红细胞多种生理生化功能。

Effect of Sargassum fusiforme Polysaccharides on Biochemistry Function of Erythrocyte Membrane in S_(180) Mice

OBJECTIVE To study the effect of Sargassum fusiforme polysaccharides(SFPS) on biochemistry function of erythrocyte membrane in S₁₈₀ mice.METHODS A tumor mouse model was developed,and the mice were treated with high,medium,and low doses of SFPS through abdominal injection for 7 d.Erythrocytes were collected and erythrocyte suspensions were prepared.Changes of Ca²⁺] in the erythrocytes of the tumor-bearing mice were measured using laser confocal scanning;changes of sialic acid and the acitivities of Na⁺,K⁺-ATPase and Ca²⁺,Mg²⁺-ATPase were meausred using relevant test kits;changes of erythrocyte membrane potential were analyzed using flow cytometry;and changes of the complex mobility of erythrocytes were measured using high performance capillary electrophoresis(HPCE).RESULTS SFPS decreased the intracelluar Ca²⁺] of erythrocytes in tumor-bearing mice,increased the content of sialic acid on surface of erythrocyte membrane,increased the acitivities of Na⁺,K⁺-ATPase and Ca²⁺,Mg²⁺-ATPase,and raised the level of erythrocyte membrane potential and the complex mobility of erythrocytes in elelctrophoresis.CONCLUSION Sargassum fusiforme polysaccharides(SFPS) can adjust or restore some biochemistry functions of erythrocyte membrane of S₁₈₀ mice.