中国HIV/AIDS患者保守表位的2F5抗体与疾病进展关系

目的 研究中国HIV/AIDS患者gp41保守表位中和抗体2F5与疾病进程的关系.方法 应用合成4(ELDKWA)-C24肽及gp41抗原肽包被96孔酶标板,ELISA方法检测特异性抗体水平.提取正常人外周血单个核细胞(PBMC),应用HIV-1 SF33毒株进行病毒多轮感染PBMC中和试验.结果 缓慢进展者(slow progressor,SP)的2F5样中和抗体水平显著高于典型进展者(typical progressor,TP)(P<0.05);2F5样中和抗体水平与gp41特异性抗体水平正相关(r=0.406,P<0.05).结论 中国HIV.-缓慢进展者体内具有高水平的2F5样抗体,2F5抗体可能是疾病不进展的保护性因素之一.

Levels of antibodies 2F5 directed against conserved epitopes in gp41 association with progression in HIV-1-infected individuals in China

Objective To study the levels of 2F5-like antibody recognized gp41 conserved epitope of HIV-1-infected individuals in China,explore the influence of 2F5-like antibody on disease progression of HIV-1 infected individuals.Methods Direct ELISA was developed for the specific comparison of levels of antibodies against gp41 and 4 (ELDKWA)-C peptides sera from HIV-1-infected individuals.Peripheral blood mononuclear cells(PBMC) were isolated from HIV-seronegative donors.Multi-round PBMC neutralization assays were performed with HIV-1 isolate SF33.The supernatant p24-Ag of HIV was analyzed.Results Levels of 2F5-like antibodies in the slow progressor (SP) plasma were significantly higher than those in typical progressor (TP)(P<0.05).There was significant correlation with the levels of 2F5 neutralizing antibody and gp41 antibodies(r=0.406,P<0.05).Conclusion The levels of 2F5-like antibodies may influence on disease progression of HIV-infected individuals.The high levels of neutralizing antibodies may arrest disease from progressing.