赖氨酸月桂基酯在小鼠活体内抗菌活性的研究

将金黄色葡萄球菌与赖氨酸月桂基酯混合后注入三组小鼠的皮下,赖酯剂量分别为186mg/kg、420mg/kg 及950mg/kg。通过观察组织切片中炎症反应程度来研究其抗菌活性。结果为:①赖酯剂量为186mg/kg 时,脓肿阳性率、脓肿大小及中性粒细胞浸润程度均低于菌感染对照组,而与青霉素治疗组相似。提示该剂量赖酯在体内有一定抗菌活性。420mg/kg 及950mg/kg 组的脓肿阳性率、脓肿大小及中性粒细胞浸润程度与菌感染对照组相似,而与青霉素治疗组的有差异。提示赖酯剂量增到420～950mg/kg时,在体内无抗菌活性。②赖酯可造成正常组织坏死,坏死率随剂量加大而增高(分别为23%,46%和61%)。③三种治疗剂量的赖酯均无明显促进肉芽组织生长的作用。

The Study of Antibacterial Activity in Vivo of Lysine Lauryl Ester (LLE) on Mice

135 mice were divided into 9 groups at random.In 3 experimental groups, animals were injected subcutaneously with Aureus Staphylococci plus saline solution of LLE 186mg/kg,420mg/kg and 950mg/kg respectively.Other 6 groups as controls,were injected respectively mith bacteria only,bacteria plus saline,bacteria plus penicillin,LLE 186mg/kg,LLE 420mg/kg and 950mg/kg.The results were as follows:(1)The percentage of abscess formation in bacteria plus saline solution of LLE 186mg/kg group was lower than that in bacteria group and bacteria plus saline group significantly,the abscess size and the extent of neutrophil infiltration in this group were smaller than that in bacteria group and bacteria plus saline group,similar to that in bacteria plus penicillin group.These results suggested that LLE 186mg/kg expressed antibacterial activity in vivo.(2)The percentages of tissue necrosis in LLE 186mg/kg group were 23%,LLE 420mg/kg group 46%,and LLE 95(mg/kg group 61%.(3)No accelerated action on the growth of granular tissue was found in any LLE groups.