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| 肺癌组织和患者体液中P_(21)~(ras)表达的初步研究 | |
| 引用本文： | 林晞,舒煦,孙泽芳,赵建升,孙芝琳. 肺癌组织和患者体液中P_(21)~(ras)表达的初步研究[J]. 四川大学学报(医学版), 1993, 0(3) |
| 作者姓名： | 林晞 舒煦 孙泽芳 赵建升 孙芝琳 |
| 作者单位： | 华西医科大学生物化学教研室(林晞，孙泽芳，赵建升)，四川省肿瘤研究所(舒煦)，华西医科大学生物化学教研室(孙芝琳) |
| 基金项目： | 国家“七五”科技攻关资助 |
| 摘 要： | 作者首次利用水平式板状凝胶电泳分离蛋白质,不经过Western转移电泳,直接用抗P~(ras)_(21)单克隆抗体在凝胶上进行酶联免疫分析,研究了人肺癌组织及患者体液中P~(ras)_(21)的表达情况。9例肺癌中8例有P~(ras)_(21)表达,其中4例明显高于正常肺;18例肺癌患者血清和2例胸水中没有检查到P~(ras)_(21)。 |
| 关 键 词： | 肺癌 P_(21)ras十二烷基硫酸钠—聚丙烯酰胺凝胶电泳 酶联免疫 基因表达 |
A Study on P_(12)~(ras) in Human Lung Cancer and Body Fluid of Cancer Patients |
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| Lin Xi,Shu Xu,Sun Zefang,Zhao Jiansheng,Sun Zhilin. A Study on P_(12)~(ras) in Human Lung Cancer and Body Fluid of Cancer Patients[J]. Journal of Sichuan University. Medical science edition, 1993, 0(3) | |
| Authors: | Lin Xi Shu Xu Sun Zefang Zhao Jiansheng Sun Zhilin |
| Affiliation: | Lin Xi;Shu Xu;Sun Zefang;Zhao Jiansheng;Sun Zhilin Department of Biochemistry |
| Abstract: | The McAb of anti-P_(12)~(ras) hasbeen used to screen 9 cases of human lungcancer tissues, 18 cases of samples and 2cases of hydrothorax samples of lung can-cer patients by means of PAGE-immuno-blots. The expression of ras gene wasfoundin 8 cases of human lung cancer amongwhich the expression of 4 cases was higherthan that of normal control. P_(12)~(ras) has not beendetected in serum and hydrothorax samplesof cancer patients. The present study indi-cated that there was P_(12)~(ras) in human lungcancer and normal control and the expres-sion level of ras gene in lung cancer wasrelated to the differentiation of cancer. |
| Keywords: | Lung cancer P_(12)~(ras) SDS-PAGE Enzyme-immunoblots Gene expression |
| 本文献已被 CNKI 等数据库收录！ | |