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水蔓菁HPLC指纹图谱研究
引用本文:王斌,伍蔚萍,冯旭,李珍,孙文基. 水蔓菁HPLC指纹图谱研究[J]. 中药材, 2004, 27(2): 95-97
作者姓名:王斌  伍蔚萍  冯旭  李珍  孙文基
作者单位:1. 西北大学生物医药重点实验室,西安,710069
2. 万荣三九药业有限公司,山西,044200
摘    要:
目的:应用RP-HPLC(DAD)建立了水蔓菁的指纹图谱.方法:用Beckman C18柱(4.6 mm×250 mm,5μm),甲醇-0.5%冰醋酸溶液恒流洗脱,流速1 ml/min,检测波长254 nm.结果:获得了该药材HPLC指纹图谱共有模式,并对不同批药材进行了相似度比较.结论:指纹图谱可用于控制水蔓菁的内在质量.

关 键 词:水蔓菁  指纹图谱  高效液相色谱
修稿时间:2003-11-02

Study on HPLC Fingerprint of Herba Veronicae Dilatatae
Wang Bin,Wu Weiping,Feng Xu,Li Zhen,Sun Wenji. Study on HPLC Fingerprint of Herba Veronicae Dilatatae[J]. Journal of Chinese medicinal materials, 2004, 27(2): 95-97
Authors:Wang Bin  Wu Weiping  Feng Xu  Li Zhen  Sun Wenji
Affiliation:Wang Bin1,Wu Weiping1,Feng Xu2,Li Zhen2,Sun Wenji1
Abstract:
Objective:To establish HPLC fingerprint of Herba Veronicae Dilatatae.Methods:Beckman C 18 column was used,with mixtures of 0.5% ice acetic acid-methanol as mobile phase.The flow rate was 1 ml/min.The wavelength of detection was 254 nm.Results:After the mutual mode of HPLC fingerprint was set up,comparsion of similar degree with the medicinal materials of different batch were carried out. Conclusion:According to the selected chromatographic conditions,a good fingerprint has been described.The method is simple,accurate and reproducible.It may have practical value for the quality control of samples of Herba Veronicae Dilatatae.
Keywords:Herba Veronicae Dilatatae  Fingerprint  HPLC  
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