| YH-16对宫颈癌细胞及其裸鼠移植瘤抑制作用研究 |
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| 引用本文： | 程丽,刘梅梅,张云艳,隋丽华.YH-16对宫颈癌细胞及其裸鼠移植瘤抑制作用研究[J].中国现代医学杂志,2008,18(10):1324-1327. |
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| 作者姓名： | 程丽 刘梅梅 张云艳 隋丽华 |
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| 作者单位： | 1. 哈尔滨医科大学附属第三医院,妇科,黑龙江,哈尔滨,150040
2. 哈尔滨医科大学附属第二医院,妇产科,黑龙江,哈尔滨,150086 |
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| 摘 要： | 目的探讨YH-16（重组内皮抑素）对宫颈癌Hela细胞及其荷瘤鼠生长抑制作用。方法用MTT法检测细胞生长;用透射电镜观察细胞凋亡、流式细胞仪检测细胞凋亡率及细胞周期;将Hela细胞移植至裸鼠皮下,观察不同浓度YH-16（0、0.4、0.75和1.5mg/kg）对荷瘤鼠皮下移植瘤生长的影响;TUNEL法观察肿瘤细胞的凋亡;免疫组化方法检测裸鼠移植瘤组织中肿瘤微血管密度（MVD）。结果YH-16抑制Hela细胞增殖（P〈0.01）;能诱导Hela细胞凋亡。YH-16能抑制裸鼠皮下移植瘤的生长（P〈0.05）;YH-1615mg/kg组的肿瘤MVD计数较对照组和其他治疗组明显降低（P〈0.05）。结论YH-16具有抑制宫颈癌Hela细胞及其皮下移植瘤生长的作用。
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| 关 键 词： | YH-16 宫颈肿瘤 异种移植模型抗肿瘤实验 肿瘤细胞 凋亡 宫颈癌细胞 裸鼠移植瘤 抑制作用 研究 nude mice tumor Hela cells growth 治疗组 对照组 计数 裸鼠皮下移植瘤 细胞增殖 结果 微血管密度 肿瘤细胞 瘤组织 免疫组化方法检测 细胞凋亡率 TUNEL |
| 文章编号： | 1005-8982(2008)10-1324-04 |
| 修稿时间： | 2007年10月15 |
Inhibition of YH-16 on growth of both Hela cells and Hela implanted tumor in nude mice |
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| CHENG Li,LIU Mei-mei,ZHANG Yun-yan,SUI Li-hua.Inhibition of YH-16 on growth of both Hela cells and Hela implanted tumor in nude mice[J].China Journal of Modern Medicine,2008,18(10):1324-1327. |
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| Authors: | CHENG Li LIU Mei-mei ZHANG Yun-yan SUI Li-hua |
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| Institution: | CHENG Li1,LIU Mei-mei2,ZHANG Yun-yan1,SUI Li-hua1 (1.Department of Gynecology,the Third Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang 150040,P.R.China,2.Department of Gynecology , Obstetrics,the Second Affiliated Hospital of Harbin Medical University,Heilongjiang 150086,P.R.China) |
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| Abstract: | Objective] To evaluate the inhibitory effect of YH-16(rhEndostatin) on the growth of cervix cancer cell lines Hela and Hela implanted tumor in nude mice. Methods] The effect of Endostatin on Hela cell proliferation was studied by means of MTT. The cell apoptosis was detected by transmission electron microscope and cell flow cytometry. The Hela cells were subcutaneously inoculated into nude mice. The therapeutic efficacy of different doses YH-16 (0, 0.4, 0.75 and 1.5 mg/kg) on bearing cancer mice was detec... |
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| Keywords: | YH-16 uterine cervix neoplasms xenograft model antitumor assays tumor cells apoptosis |
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