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| 丙型肝炎病毒结构蛋白对病毒细胞间传播的影响 | |
| 引用本文： | 赵婷1,邢国珍1,徐朝1,龙钢2,许君1. 丙型肝炎病毒结构蛋白对病毒细胞间传播的影响[J]. 现代预防医学, 2016, 0(16): 2997-3001 |
| 作者姓名： | 赵婷1 邢国珍1 徐朝1 龙钢2 许君1 |
| 作者单位： | 1. 河南农业大学生命科学学院，河南 郑州 450002；2. 中国科学院上海巴斯德研究所，上海 200031 |
| 摘 要： | 目的 探究丙型肝炎病毒（Hepatitis C Virus, HCV）结构蛋白对病毒细胞间传播的影响。方法 分别以HCV基因组电转后的Huh7.5.1细胞和未经电转的Huh7.5.1细胞作为供体细胞和受体细胞，同时加入中和抗体，建立HCV细胞间传播检测系统，应用已建立好的细胞间传播检测系统探究病毒结构蛋白对病毒细胞间传播的影响。结果 在HCV细胞间传播检测系统中，野生型病毒可进行正常的细胞间传播，结构蛋白缺失的缺陷型病毒不能进行细胞间传播。结论 HCV的结构蛋白对HCV进行细胞间传播至关重要。 |
| 关 键 词： | 丙型肝炎病毒 结构蛋白 细胞间传播 |
The effect of HCV structural proteins on HCV cell-to-cell transmission |
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| ZHAO Ting,XING Guo-zhen,XU Zhao,LONG Gang,XU Jun. The effect of HCV structural proteins on HCV cell-to-cell transmission[J]. Modern Preventive Medicine, 2016, 0(16): 2997-3001 | |
| Authors: | ZHAO Ting XING Guo-zhen XU Zhao LONG Gang XU Jun |
| Affiliation: | *Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China |
| Abstract: | Objective The effect of hepatitis C virus (HCV) structural proteins on HCV cell-to-cell transmission were analyzed in this study. Methods HCV cell-to-cell transmission system was established. Huh7.5.1 cells electroporated with HCV genome were used as donor cells. Naive Huh7.5.1 cells were used as recipient cells. Donor cells were mixed with recipient cells and HCV neutralizing antibody to neutralize cell-free HCV virus. Results By HCV cell-to-cell transmission system, it was found that the wild type HCV could naturally spread by cell-to-cell transmission, while the structural proteins-deficient virus cannot. Conclusion The results suggested that the structural proteins were essential to HCV cell-to-cell transmission. |
| Keywords: | Hepatitis C virus (HCV) Structural proteins Cell-to-cell transmission |
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