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| 高效液相色谱法测定广东产白花蛇舌草中槲皮素含量 | |
| 引用本文： | 朱缨,王琳,朱磊,吴健. 高效液相色谱法测定广东产白花蛇舌草中槲皮素含量[J]. 中国药业, 2010, 19(18): 23-24 |
| 作者姓名： | 朱缨 王琳 朱磊 吴健 |
| 作者单位： | 1. 苏州卫生职业技术学院药学教研室,江苏,苏州,215009 2. 江苏省吴江第一人民医院药剂科,江苏,吴江,215200 3. 南京农业大学园艺学院中药材科学系,江苏,南京,210095 |
| 摘 要： | 目的采用高效液相色谱（HPLC）法测定广东产白花蛇舌草的槲皮素含量。方法色谱柱为Zorbax SB-C18柱（250 mm×4.6 mm,5.0μm）,流动相为乙腈-0.2%磷酸（30∶70）,检测波长370 nm,流速1 mL/min。结果槲皮素进样量在0.05～0.5μg范围内与峰面积线性关系良好（r=0.999 9）,平均加样回收率为100.19%,RSD为0.81%（n=6）。结论该法快速、简便、准确,为评价白花蛇舌草的质量提供了科学依据。 |
| 关 键 词： | 白花蛇舌草 广东 槲皮素 高效液相色谱法 |
Content Determination of Quercetin in Oldenlandia Diffusa (Willd.) Roxb from Guangdong by HPLC |
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| Zhu Ying,Wang Lin,Zhu Lei,Wu Jian. Content Determination of Quercetin in Oldenlandia Diffusa (Willd.) Roxb from Guangdong by HPLC[J]. China Pharmaceuticals, 2010, 19(18): 23-24 | |
| Authors: | Zhu Ying Wang Lin Zhu Lei Wu Jian |
| Affiliation: | g First People＇ s Hospital, Wujiang, Jiangsu, China 215200; 3. Department of Chinese Herbal Technology, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, China 210095) |
| Abstract: | Objective To determine the content of quercetin in Oldenlandia diffusa （Willd.） from Guangdong. Methods The analysis was performed on the HPLC C18 column（250 mm ×4.6mm,5.0 μm） at the detection wavelength of 370 nm. The mobile phase consisted of acetonitrile -0.2% phosphoric acid （30：70） with the flow rate of 1 mL/min. Results The calibration curve was linear in the range of 0.05-0.5μg for quercetin （r=0.999 9）.The average recovery rate was 100. 19% with RSD was 0.81% （n=6）.Conclusion The method is simple,rapid and accurate,which could be used for the quality control of Oldenlandia diffusa （Willd.）. |
| Keywords: | Oldenlandia diffusa （Willd.） Guangdong quercetin HPLC |
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