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| Necrostatin-1对6-OHDA致PC12细胞氧化应激损伤的影响 | |
| 引用本文： | 周生奎,王杰,程言博,徐兴顺,耿德勤.Necrostatin-1对6-OHDA致PC12细胞氧化应激损伤的影响[J].徐州医学院学报,2010,30(8). |
| 作者姓名： | 周生奎 王杰 程言博 徐兴顺 耿德勤 |
| 作者单位： | 1. 徐州医学院研究生学院2007级,江苏,徐州,221002;徐州医学院附属医院神经内科,江苏,徐州,221002 2. 徐州医学院药学院,江苏,徐州,221004 3. 徐州医学院附属医院神经内科,江苏,徐州,221002 |
| 摘 要： | 目的研究Necrostatin-1(Nec-1)对帕金森病(Parkinson's disease,PD)细胞模型线粒体失能的影响,探讨Nec-1在帕金森病发病机制中的作用。方法经不同浓度Nec-1预处理的PC12细胞加入6-OHDA建立多巴胺能神经元损伤模型。用4-甲基偶氮唑蓝(MTT)法检测PC12细胞活力,JC-1试剂盒检测线粒体膜电位,免疫印迹法检测Cathepsin B与Bcl-2蛋白表达。结果 100μmol/L的6-OHDA处理PC12细胞24h,细胞活力较正常对照组明显降低(P<0.05);与模型组比较,5、10、20、30、60μmol/L的Nec-1可明显减轻6-OHDA的毒性,90μmol/L的Nec-1可加重6-OHDA的毒性。Nec-1可阻抑线粒体膜电位下降。与正常对照组相比,模型组Cathepsin B表达明显增加而Bcl-2的表达显著降低(P<0.05)。与模型组相比,Nec-1与UTI可降低Cathepsin B表达并使Bcl-2的表达逐渐增加(P<0.05)。结论 Nec-1在一定的浓度范围内可提高PC12细胞存活力,通过稳定线粒体膜电位、抑制Cathepsin B的表达、上... |
| 关 键 词： | Necrostatin-1 6-羟基多巴胺 PC12细胞 帕金森病 线粒体失能 |
Effects of necrostatin-1 on the 6-hydroxydopamine-induced oxidative stress damage in PC12 cells |
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| ZHOU Shengkui,WANG Jie,CHENG Yanbo,XU Xingshun,GENG Deqin.Effects of necrostatin-1 on the 6-hydroxydopamine-induced oxidative stress damage in PC12 cells[J].Acta Academiae Medicinae Xuzhou,2010,30(8). | |
| Authors: | ZHOU Shengkui WANG Jie CHENG Yanbo XU Xingshun GENG Deqin |
| Institution: | 1.Grade 2007; Graduate School; Xuzhou Medical College; Xuzhou; Jiangsu 221002; China; 2.School of Pharmacy; Jiangsu 221004; 3.Department of Neurology; The Affiliated Hospital of Xuzhou Medical College; Jiangsu 221002); |
| Abstract: | Objective To investigate the effect of necrostatin-1 (Nec-1) in mitochondrial dysfunction of catecholaminergic cell line PC-12 induced 6-hydroxydopamine (6-OHDA) and discuss the possible role of Nec-1 in the molecular mechanisms of the pathogenesis of Parkinson s disease (PD) .Methods Dopaminergic neuronal injury model was induced by the addition of 6-OHDA into PC12 cells cultured in vitro and pretreated at different concentrations of Nec-1.Cell viability,mitochondrial membrane potential (MMP) and the expre... |
| Keywords: | Necrostatin-1 |
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