| 四种快速检测大肠杆菌O_(157):H_7的方法研究 |
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| 引用本文： | 郭惠,杨晋川,张雷,许静静,王路梅,杜阳光.四种快速检测大肠杆菌O_(157):H_7的方法研究[J].中国卫生检验杂志,2011(5). |
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| 作者姓名： | 郭惠 杨晋川 张雷 许静静 王路梅 杜阳光 |
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| 作者单位： | 江苏省徐州市疾病预防控制中心; |
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| 基金项目： | 江苏省预防医学科研课题(Y200722) |
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| 摘 要： | 目的:将磁免疫分离技术(immunomagnetic separation,IMS)与显色培养(chromogenic media,Ch)和荧光定量(Re-al-time,R-t)PCR技术结合建立快速检测肉制品中EHECO157:H7的方法。方法:应用四种快速检测方法检测模拟样品和423份肉制品中的EHECO157:H7,比较不同方法的灵敏度和特异度。结果:IMS-Ch法特异性最好,24 h对目标菌含量1-5 CFU/10 g的样品检出率为95%。IMS-PCR对实际样品检测效果评价的尤等指数最高。结论:IMS-PCR是一种可信的快速筛选方法,IMS-Ch法为灵敏、快速的确证方法。
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| 关 键 词： | EHECO157:H7 免疫磁珠捕获 荧光定量PCR 显色培养基 |
Comparison of Four Metods in Rapid detection of Enterohemorrhagic Escherichia coli O_(157):H_7 strain |
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| GUO Hui,YANG Jin-chuan,ZHANG Lei,XU Jing-jing,WANG Lu-mei,DU Yang-guang.Comparison of Four Metods in Rapid detection of Enterohemorrhagic Escherichia coli O_(157):H_7 strain[J].Chinses Journal of Health Laboratory Technology,2011(5). |
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| Authors: | GUO Hui YANG Jin-chuan ZHANG Lei XU Jing-jing WANG Lu-mei DU Yang-guang |
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| Institution: | GUO Hui,YANG Jin-chuan,ZHANG Lei,XU Jing-jing,WANG Lu-mei,DU Yang-guang(Xuzhou Center for Diseade Control and Prevention,Xuzhou 221006,China) |
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| Abstract: | Objective:To develop a method for the rapid detection of EHECO157:H7,Immunomagnetic separation technique were carried out to capture pathogens and extract DNA,this assay combined with fluorescence PCR and chromogenic media technique.Methods: Four methods in rapid detection were used to detcect EHECO157:H7 strain in meat products,the sensitivity and specificity was evaluated and compared.Results: It was demonstrated that IMS-Ch assay was proved to be 100% specific.When the bacteria concentration was as low a... |
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| Keywords: | EHECO157:H7 Immunomagnetic separation Fluorescence PCR Chromogenic media |
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