| 培养温度对多拷贝毕赤酵母猪胰岛素前体的分泌表达和生理特性影响 |
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| 引用本文： | 王玥,杭海峰,郭美锦,朱泰承,Tadayuki Imanak,庄英萍.培养温度对多拷贝毕赤酵母猪胰岛素前体的分泌表达和生理特性影响[J].医学教育探索,2012(1):45-52. |
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| 作者姓名： | 王玥 杭海峰 郭美锦 朱泰承 Tadayuki Imanak 庄英萍 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237 |
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| 基金项目： | National Natural Science Foundation of China(3056006); Open Funding Project of the State Key Laboratory of Bioreactor Engineering(2060204). |
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| 摘 要： | 研究了培养温度对多拷贝毕赤酵母分泌表达猪胰岛素前体（PIP）的生理特性的影响。将多拷贝重组菌A2（12拷贝）和A3（18拷贝）分别在25 ℃和 30 ℃培养，25 ℃培养时PIP表达水平分别比30 ℃培养时提高了40%和32%，而A3细胞的存活率提高了20%。对A3细胞内相关蛋白折叠和氧化程度的KAR2，PDI1, GLR和TRR1基因转录水平分析发现，相比30 ℃培养25 ℃培养时减少了16%~35%，这表明多拷贝重组A3菌株在低温培养时比高温培养在蛋白折叠和氧化协廹响应有明显的减缓效应，从而导致外源蛋白表达提高了40%.
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| 关 键 词： | 毕赤酵母 基因拷贝数 转录分析 猪胰岛素前体 培养温度 |
Effect of Temperature on the Secretory Expression of Porcine
Insulin Precursor and Physiological Characterization of
Multi copy P. pastoris |
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| WANG Yue,HANG Hai-feng,GUO Mei-jin,ZHU Tai-cheng,Tadayuki Imanaka and ZHUANG Ying-ping.Effect of Temperature on the Secretory Expression of Porcine
Insulin Precursor and Physiological Characterization of
Multi copy P. pastoris[J].Researches in Medical Education,2012(1):45-52. |
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| Authors: | WANG Yue HANG Hai-feng GUO Mei-jin ZHU Tai-cheng Tadayuki Imanaka and ZHUANG Ying-ping |
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| Institution: | State Key Laboratory of Bioreactor Engineering, East China University of
Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of
Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of
Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of
Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of
Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of
Science and Technology, Shanghai 200237, China |
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