SVF细胞影响移植脂肪LPL表达的实验研究

目的 探讨脂肪移植后脂蛋白脂肪酶(Lipoprotein lipase,LPL)在SVF(Stromal vascular fraction)辅助移植脂肪组织中的表达模式。方法 本实验以新鲜分离的自体SVF细胞辅助移植的脂肪组织为实验组,以脂肪组织与等量生理盐水的混合物为对照组,随机在裸鼠背部两侧注射建立动物模型,对两组移植物进行脂周蛋白免疫组化染色分析、LPL免疫组化染色分析和LPL蛋白含量测定。结果 TPL蛋白含量测定及LPL免疫组化染色分析显示,随着移植时间的延长,实验组和对照组LPL表达逐渐下降,在第2周、第3周、第4周时,实验组LPL表达明显高于对照组。脂周蛋白免疫组化染色分析显示,两组脂肪移植后的存活细胞均逐渐减少,但第2周、第3周、第4周实验组存活细胞数高于对照组。结论 SVF促进脂肪组织表达LPL,表达差异在移植后的第2周开始出现,这可能与SVF提高脂肪组织存活率和ASCs(Adipose-derived stromal cells)成脂分化有关。

Effect of SVF Cells on the LPL Expression after Fat Transplantation

Objective To explore the effect of adipose stromal vascular fraction （SVF） cells on the LPL expression after fat transplantation. Methods Freshly isolated SVFs （experimental group） were mixed with adipose tissue and then injected under the back skin of nudes randomly on one side. In the same manner, the mixture of physiological saline and adipose tissue （control group） were injected on the other side. Immunohistochemistry for perilipin, immunohistochemistry for LPL and western blot for LPL were combined to explore the trends of LPL expression at 1, 2, 3 and 4 weeks. Results According to the results of western blot and immunohistology of LPL, the levels of LPL started to decrease as early as the transplantation in both groups. And the expression of LPL in the experimental group was higher than the control group at 2, 3 and 4 weeks after implantation. Immunohistology of perilipin indicated no remarkable changes in the viable adipocyte zone during the first week. The adipose cells decreased day to day in both group. And 2, 3 and 4 weeks after implantation there were more survived adipose cell in the experimental group. Conclusion SVF can improve the expression of LPL in transplanted adipose tissue. It begins to make effects at the second week post-implantation. It may be related to its effect on the adipose cell survival and the adipogenic differentiation of ASCs.