| HPLC法测定乳酸左氧氟沙星注射液左氧氟沙星含量 |
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| 引用本文： | 孔薇. HPLC法测定乳酸左氧氟沙星注射液左氧氟沙星含量[J]. 安徽医药, 2010, 14(10): 1151-1152. DOI: 10.3969/j.issn.1009-6469.2010.10.014 |
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| 作者姓名： | 孔薇 |
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| 作者单位： | 安徽医科大学附属第三医院;合肥市第一人民医院药学部,安徽,合肥,230061 |
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| 基金项目： | 安徽省第三周期临床医学特色专科基金，合肥市第二周期医学重点学科基金 |
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| 摘 要： | 目的建立乳酸左氧氟沙星注射液中左氧氟沙星含量测定方法。方法采用高效液相色谱法,VP-ODS（4.6 mm×150mm,5μm）;流动相：三乙胺磷酸溶液-乙腈（82：18）;流速：1.0 ml·min^-1;柱温：40℃;检测波长为286 nm。供试品溶液制备采用流动相溶解并稀释成每1ml中约含0.1mg的溶液。结果左氧氟沙星线性范围为1.73～15.61 mg·L^-1,平均加样回收率为100.21%。结论本法简捷,快速可靠,可用于控制制剂质量。
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| 关 键 词： | 高效液相色谱 乳酸左氧氟沙星注射液 左氧氟沙星 |
Determination of levofloxacin in levofloxacin lactate injection by HPLC |
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| KONG Wei. Determination of levofloxacin in levofloxacin lactate injection by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2010, 14(10): 1151-1152. DOI: 10.3969/j.issn.1009-6469.2010.10.014 |
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| Authors: | KONG Wei |
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| Affiliation: | KONG Wei(Department of Pharmacy,The Third Hospital Affiliated to Anhui Medical University,Hefei First People’s Hospital,Hefei 230061) |
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| Abstract: | Aim To develop a method for determining the contents of levofloxacin in levofloxacin lactate injection.Methods The analyt-ical column was a Hypersil VP-ODS(4.6 mm × 250 mm,5 μm).The mobile phase consisted of triethanolamine-methyl cyanides(82: 18).The flow rate was 1.0 ml.min-1and the column temperature was 40℃.The detection wavelengths of luteolin was at 286 nm.Re-sults The calibration curves of luteolin was in good linearity over the rangs from 1.73 to 15.61 mg.L-1and the average recovery was 100.21%.Conclusion The method is simple,rapid,reliable and can be used for the drug control. |
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| Keywords: | HPLC levofloxacin lactate injection levofloxacin |
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