绞股蓝总皂苷对血管性痴呆大鼠海马一氧化氮合酶及核酸的保护作用

目的 观察绞股蓝总皂苷(GP)对血管性痴呆(VD)大鼠海马神经元型一氧化氮合酶(nNOS)及核酸的保护作用。方法 采用改良的Pulsinelli 4-血管阻断(4-VO)方法建立大鼠VD模型，用免疫组化法研究GP对大鼠海马nNOS的表达的影响，并用吖啶橙染色法进行GP对VD大鼠海马的DNA和RNA保护作用的研究。结果 与正常对照组比较，VD大鼠海马CA1区和CA3区nNOS阳性神经元数目明显减少，而DNA和RNA吖啶橙染色后的荧光强度(反映DNA和RNA含量)明显减弱，GP 200 mg／kg ig给药组海马各亚区的nNOS阳性神经元神经细胞数比VD模型组明显增多。DNA和RNA吖啶橙染色后的荧光强度强于VD模型组，与正常对照组相似。结论 GP可明显增强VD大鼠海马nNOS阳性神经元的表达，并对DNA和RNA有保护作用。

Protective effect of gypenosides on expression of neuronal nitric oxide synthase and nucleic acid in hippocampus of vascular dementia rat

Object To observe the protective effect of gypenosides (GP) on the expression of neuronal niteic oxide synthase (nNOS) and the nucleic acid of hippocampus in vascular dementia (VD) of rats. Methods Modified Pulsinelli's 4 vessel occlusion (4 VO) method was used to establish the models of VD in rats. The immunohistochemical method and acridine orange (AO) staining method were used to investigate the expression of nNOS and the DNA and RNA contents in different hippocampus subfields in VD rats. Results The number of nNOS positive neurons decreased significantly and the fluorescent intensity (reflecting DNA and RNA contents) of the DNA and RNA abated markedly in the areas of hippocampus of VD group compared with the normal control group. In the group of ig GP (200 mg/kg), the number of nNOS positive neurons increased significantly and the fluorescent intensity of the DNA and RNA enhanced markedly compared with the model group and was the same as the control group. Conclusion It is shown that the expression of nNOS could be increased significantly and the injury of nucleic acid decreased in hippocampus of VD rats by GP.