人胎肾上腺,肝和肾内谷胱甘肽相关酶活性

目的：通过谷胱甘肽相关酶活性及其在亚细胞分布，了解胎肾上腺在发育期间对活性代谢产物的解毒处理能力。方法：制备肾上腺、肝亚细胞组分。测定谷胱甘肽转移酶（ＧＳＴ）、还原酶、过氧化物酶。结果：ＧＳＴ在胎肾上腺微粒体、线粒体、胞浆中含量分别是肝各亚细胞组分中的３７３％、２７０％和民６７％、肾上腺微粒体ＧＳＴ活性与细胞色素Ｐ－４５０、与氨基比林脱甲基酶活性皆呈正相关，肾上腺线粒体谷胱甘肽还原酶、过氧化物分别

Glutathione-related enzyme activities in human fetal adrenal, liver, and kidney.

AIM: To understand the capacity of fetal adrenal to catalyze reaction metabolites. METHODS: Subcellular fractions were prepared by differential centrifugation in fetal adrenal and liver. Glutathione (GSH)-transferase, reductase, and peroxidase were measured. RESULTS: The mean values (mumol.min-1/g protein) of GSH-transferase activities in adrenal microsome (112 +/- 34), mitochondria (62 +/- 35), and cytosol (191 +/- 89) were 373%, 270%, and 167%, respectively, higher than those in the corresponding fractions of fetal liver. Adrenal microsomal GSH-transferase was positively correlated with adrenal microsomal P-450 (r = 0.821, P < 0.01), and with adrenal microsomal aminopyrine N-demethylase (r = 0.829, P < 0.01). The GSH reductase contents (mumol.min-1/g protein) in adrenal mitochondria (24 +/- 14), and in S9 (36 +/- 15) were almost 5 times higher, compared with that in liver. Selenium-dependent GSH peroxidase was present in all the adrenal. CONCLUSION: Fetal adrenal, with greater capacities than those of liver in detoxifying reaction, may act as a drug-metabolizing organ during development.